Source:http://linkedlifedata.com/resource/pubmed/id/11826978
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
2002-2-5
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pubmed:abstractText |
Hydrogenovibrio marinus was suggested to have only membrane-bound hydrogenase (MBH). The change of cultivation pO2 did not affect the molecular species of hydrogenase expressed. We propose the MBH is grouped in class I [NiFe] MBH according to the subunit composition, size (Mw 38,000 and Mw 74,000 subunits) and N-terminal sequences of the subunits, and arrangement of the structural genes. Ni-requirement for the autotrophic growth on H2 also suggested the MBH is the Ni-containing type. Southern hybridization analysis using a part of the MBH gene showed a possibility of the presence of two highly homologous MBHs which were not separated by SDS-PAGE.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0916-8451
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2780-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11826978-Amino Acid Sequence,
pubmed-meshheading:11826978-Base Sequence,
pubmed-meshheading:11826978-Blotting, Southern,
pubmed-meshheading:11826978-DNA, Bacterial,
pubmed-meshheading:11826978-DNA Primers,
pubmed-meshheading:11826978-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11826978-Hydrogen,
pubmed-meshheading:11826978-Hydrogenase,
pubmed-meshheading:11826978-Molecular Sequence Data,
pubmed-meshheading:11826978-Oxidation-Reduction,
pubmed-meshheading:11826978-Proteobacteria,
pubmed-meshheading:11826978-Sequence Homology, Amino Acid
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pubmed:year |
2001
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pubmed:articleTitle |
Analysis of the molecular species of hydrogenase in the cells of an obligately chemolithoautotrophic, marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus.
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pubmed:affiliation |
Department of Bioresource Science, College of Agriculture, Ibaraki University, Japan. hiro@msv.ipc.ibaraki.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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