Source:http://linkedlifedata.com/resource/pubmed/id/11825597
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2002-2-4
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pubmed:abstractText |
Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs within certain flanking base pairs are recognized as a danger signal by the innate immune system of vertebrates. Using lymphocyte proliferative response (LPR) and IFN-gamma secretion assays, a panel of 38 ODN was screened for immunostimulatory activity on bovine peripheral blood mononuclear cells. ODN composed of a nuclease resistant phosphorothioate backbone and a leading 5'-TCGTCGTT-3' motif with two 5'-GTCGTT-3' motifs were highly stimulatory in both assays. Flow cytometric analysis and cell-specific surface marker labeling determined that B-cells (surface IgM(+)) were the primary cell population responding in the LPR assay. Depletion of T cells (CD3(+)) from the PBMC population did not affect IFN-gamma secretion or B-cell proliferation when cultured with CpG-ODN. However, depletion of monocytes (DH59B(+)) completely abrogated the ability of CpG-ODN to stimulate IFN-gamma secretion, and significantly reduced the B-cell proliferative response. These data establish the identity of an optimal immunostimulatory CpG motif for cattle and demonstrate that monocytes play a pivotal role in the ability of cell populations to respond to CpG-ODN. These data provide insight for future studies investigating the mechanism of CpG-ODN bioactivity and its application in novel vaccine formulations and immunotherapy.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0165-2427
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43-59
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11825597-Adjuvants, Immunologic,
pubmed-meshheading:11825597-Animals,
pubmed-meshheading:11825597-Cattle,
pubmed-meshheading:11825597-Female,
pubmed-meshheading:11825597-Immunophenotyping,
pubmed-meshheading:11825597-Interferon-gamma,
pubmed-meshheading:11825597-Leukocytes, Mononuclear,
pubmed-meshheading:11825597-Lymphocyte Activation,
pubmed-meshheading:11825597-Male,
pubmed-meshheading:11825597-Monocytes,
pubmed-meshheading:11825597-Oligodeoxyribonucleotides,
pubmed-meshheading:11825597-T-Lymphocytes
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pubmed:year |
2002
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pubmed:articleTitle |
Monocytes are required for optimum in vitro stimulation of bovine peripheral blood mononuclear cells by non-methylated CpG motifs.
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pubmed:affiliation |
Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Sask., S7N 5E3, Saskatoon, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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