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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-1-31
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pubmed:abstractText |
Since retinoic acid receptor (RAR)-beta mRNA is frequently lost during esophageal carcinogenesis and esophageal cancer cells that do not express RAR-beta are resistant to retinoic acid (RA), we stably transfected RAR-beta expression vector into an esophageal cancer cell line TE-8 and an antisense RAR-beta into TE-3 cells. Transfection of RAR-beta decreased cell growth and colony formation and induced apoptosis in TE-8 cells. Antisense RAR-beta-transfected TE-3 cells had a shorter doubling time and became resistant to RA. Induction of RAR-beta decreased COX-2 expression in RAR-beta transfected TE-8 cells, whereas antisense RAR-beta transfected TE-3 cells increased COX-2 expression. The inhibitory effect of RAR-beta on COX-2 expression was further enhanced in the presence of RA, which was blocked by an RAR antagonist. The synthetic retinoid N-(4-hydroxyphenyl)retinamide, which does not bind effectively to RAR-beta, had no effect on COX-2 suppression. Furthermore, RA blocked bile acid-induced COX-2 expression and prostaglandin E(2) production only in the RAR-beta positive cells. Our data demonstrated that anticancer effect of RAR-beta may be related to its ability to suppress COX-2 expression and support that the loss of RAR-beta expression may contribute to esophageal carcinogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bile Acids and Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/retinoic acid receptor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
411-8
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11821953-Apoptosis,
pubmed-meshheading:11821953-Bile Acids and Salts,
pubmed-meshheading:11821953-Blotting, Western,
pubmed-meshheading:11821953-Cell Division,
pubmed-meshheading:11821953-Cell Survival,
pubmed-meshheading:11821953-Cyclooxygenase 1,
pubmed-meshheading:11821953-Cyclooxygenase 2,
pubmed-meshheading:11821953-DNA, Antisense,
pubmed-meshheading:11821953-Dinoprostone,
pubmed-meshheading:11821953-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11821953-Esophageal Neoplasms,
pubmed-meshheading:11821953-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11821953-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11821953-Humans,
pubmed-meshheading:11821953-Isoenzymes,
pubmed-meshheading:11821953-Membrane Proteins,
pubmed-meshheading:11821953-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:11821953-Receptors, Retinoic Acid,
pubmed-meshheading:11821953-Transfection,
pubmed-meshheading:11821953-Tretinoin,
pubmed-meshheading:11821953-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Induction of retinoic acid receptor-beta suppresses cyclooxygenase-2 expression in esophageal cancer cells.
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pubmed:affiliation |
Department of Clinical Cancer Prevention, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, TX 77030, USA.
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pubmed:publicationType |
Journal Article
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