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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-1-31
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pubmed:abstractText |
An unnatural base pair of 2-amino-6-(2-thienyl)purine (denoted by s) and pyridin-2-one (denoted by y) was developed to expand the genetic code. The ribonucleoside triphosphate of y was site-specifically incorporated into RNA, opposite s in a template, by T7 RNA polymerase. This transcription was coupled with translation in an Escherichia coli cell-free system. The yAG codon in the transcribed ras mRNA was recognized by the CUs anticodon of a yeast tyrosine transfer RNA (tRNA) variant, which had been enzymatically aminoacylated with an unnatural amino acid, 3-chlorotyrosine. Site-specific incorporation of 3-chlorotyrosine into the Ras protein was demonstrated by liquid chromatography-mass spectrometry (LC-MS) analysis of the products. This coupled transcription-translation system will permit the efficient synthesis of proteins with a tyrosine analog at the desired position.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-chlorotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bacteriophage T7 RNA polymerase
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1087-0156
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pubmed:author |
pubmed-author:FujiwaraTsuyoshiT,
pubmed-author:HiraoIchiroI,
pubmed-author:KigawaTakanoriT,
pubmed-author:KodamaKoichiroK,
pubmed-author:MitsuiTsuneoT,
pubmed-author:NakayamaHiroshiH,
pubmed-author:NishikawaKazuyaK,
pubmed-author:OhtsukiTakashiT,
pubmed-author:OkuniTaekoT,
pubmed-author:TakioKojiK,
pubmed-author:YabukiTakashiT,
pubmed-author:YokogawaTakashiT,
pubmed-author:YokogawaTomokoT,
pubmed-author:YokoyamaShigeyukiS
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-82
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11821864-Amino Acid Sequence,
pubmed-meshheading:11821864-Amino Acids,
pubmed-meshheading:11821864-Base Sequence,
pubmed-meshheading:11821864-Cell-Free System,
pubmed-meshheading:11821864-Codon,
pubmed-meshheading:11821864-DNA,
pubmed-meshheading:11821864-DNA-Directed RNA Polymerases,
pubmed-meshheading:11821864-Escherichia coli,
pubmed-meshheading:11821864-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:11821864-Genetic Variation,
pubmed-meshheading:11821864-Models, Chemical,
pubmed-meshheading:11821864-Molecular Sequence Data,
pubmed-meshheading:11821864-Plasmids,
pubmed-meshheading:11821864-Protein Biosynthesis,
pubmed-meshheading:11821864-Protein Engineering,
pubmed-meshheading:11821864-RNA, Messenger,
pubmed-meshheading:11821864-RNA, Transfer,
pubmed-meshheading:11821864-Time Factors,
pubmed-meshheading:11821864-Transcription, Genetic,
pubmed-meshheading:11821864-Tyrosine,
pubmed-meshheading:11821864-Viral Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
An unnatural base pair for incorporating amino acid analogs into proteins.
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pubmed:affiliation |
Yokoyama CytoLogic Project, ERATO, JST, c/o RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. ihirao@postman.riken.go.jp
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pubmed:publicationType |
Journal Article
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