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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-3-6
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pubmed:abstractText |
In mammals, sequence-specific termination of DNA replication within the ribosomal RNA genes is catalyzed by a defined DNA-protein complex that includes transcription termination factor I (TTF-I). Here we show that TTF-I acts as a polar contrahelicase contrary to the intrinsic 3' -->5' helicase activity of SV40 large T antigen. The contrahelicase activity requires binding of TTF-I to its cognate recognition site and the presence of an auxiliary GC-rich sequence, which is able to form a specific secondary structure. Mutations in the GC-rich sequence lead to a loss of folding into correct secondary structure and abrogate contrahelicase activity. The finding suggests that a specific interaction between the Sal box-bound TTF-I and the GC-rich sequence is essential for the inhibition of T antigen helicase. Analyses of N-terminally truncated mutants of TTF-I showed inhibition of helicase by the same domain of TTF-I, which is also responsible for replication fork arrest.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-10640269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-10717381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-1454828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-1849268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-1988950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-2826443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-2826446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-3019672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-3375241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-4075406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-8955146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-9267035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11818337-9514756
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1469-221X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2002
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pubmed:articleTitle |
Transcription termination factor TTF-I exhibits contrahelicase activity during DNA replication.
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pubmed:affiliation |
Institute of Biochemistry, University of Würzburg, D-97074 Würzburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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