Linked Life Data

11817729

Source:http://linkedlifedata.com/resource/pubmed/id/11817729

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-1-30
pubmed:abstractText
A low-capacity cation-exchange column was newly developed for the separation of amino acids. A highly cross-linked macro-porous polystyrene-divinylbenzene co-polymer was functionalized by a sulfoacylation reaction. The exchange capacity was controllable at the acylation step. The capacity between 55 and 60 micromol/column was adequate for the practical separations in acceptable retention times. The 5-microm base polymers having average pore diameters smaller than 3 nm gave satisfactory results, and those having 1.5-nm pore was most favorable. Several isocratic elution conditions at different pH values adjusted by phosphate buffer of mM order with or without acetonitrile could provide good separations for individual classes of amino acids, i.e., acidic, neutral, hydrophobic, and basic groups. The results provided fundamental data for constructing gradient elution systems required for the simultaneous separation of protein amino acids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0910-6340
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-63
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Sulfoacylated macro-porous polystyrene-divinylbenzene low-capacity cation exchanger selective for amino acids.
pubmed:affiliation
Department of Analytical Chemistry, Faculty of Engineering, Yokohama National University, Hodogaya, Japan.
pubmed:publicationType
Journal Article