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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2002-4-1
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pubmed:abstractText |
Expression of the human cyclin-dependent protein kinase inhibitor p57(Kip2) gene was previously shown to be specifically and strongly activated by the retroviral trans-activator Bel1 of human foamy virus by means of expression profiling, Northern, and Western blot analysis. Here we report that Bel1-mediated trans-activation was conferred by a Bel1 response element (BRE) located in the second exon of p57(Kip2). The intragenic Kip2-BRE was capable of trans-activating the luciferase reporter gene upon cotransfection with Bel1. In electrophoretic mobility shift assays using 293T nuclear extracts or a purified glutathione S-transferase (GST) small middle dotBel1 fusion protein, we identified the 55-nucleotide-long Kip2-BRE site that mainly consists of three direct repeats of 14-mers partially homologous to a functionally active BRE in the viral internal promoter. The specificity of the transactivator-DNA binding was shown by using mutated and shortened Kip2-BRE oligodeoxynucleotides in competition experiments with the authentic viral internal promoter and by Bel1-specific antibody that led to a supershift of the nuclear protein small middle dotKip2-BRE and GST small middle dotBel1 small middle dotKip2-BRE complex. The data indicate that Bel1 can directly bind to BRE sites. The cellular Kip2-BRE can be used to predict those human genes that are directly or indirectly activated by the Bel1 trans-activator.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1C protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/bel1 protein, Human foamy virus
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12032-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11815601-Base Sequence,
pubmed-meshheading:11815601-Binding, Competitive,
pubmed-meshheading:11815601-Binding Sites,
pubmed-meshheading:11815601-Cell Line,
pubmed-meshheading:11815601-Cell Nucleus,
pubmed-meshheading:11815601-Cyclin-Dependent Kinase Inhibitor p57,
pubmed-meshheading:11815601-DNA,
pubmed-meshheading:11815601-DNA-Binding Proteins,
pubmed-meshheading:11815601-Exons,
pubmed-meshheading:11815601-Genes, Reporter,
pubmed-meshheading:11815601-Glutathione Transferase,
pubmed-meshheading:11815601-Humans,
pubmed-meshheading:11815601-Immunoblotting,
pubmed-meshheading:11815601-Luciferases,
pubmed-meshheading:11815601-Models, Genetic,
pubmed-meshheading:11815601-Molecular Sequence Data,
pubmed-meshheading:11815601-Nuclear Proteins,
pubmed-meshheading:11815601-Plasmids,
pubmed-meshheading:11815601-Promoter Regions, Genetic,
pubmed-meshheading:11815601-Protein Binding,
pubmed-meshheading:11815601-Recombinant Fusion Proteins,
pubmed-meshheading:11815601-Retroviridae Proteins,
pubmed-meshheading:11815601-Trans-Activators,
pubmed-meshheading:11815601-Transcription, Genetic,
pubmed-meshheading:11815601-Transcriptional Activation,
pubmed-meshheading:11815601-Transfection
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pubmed:year |
2002
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pubmed:articleTitle |
Identification and functional characterization of an intragenic DNA binding site for the spumaretroviral trans-activator in the human p57Kip2 gene.
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pubmed:affiliation |
Division of Retroviral Gene Expression, Research Program Applied Tumor Virology, German Cancer Research Center, Im Neuenheimer Feld 242, 69009 Heidelberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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