Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases alpha and beta with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.
Department of Applied Biological Science, Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
