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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-1-29
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pubmed:abstractText |
The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial cell culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP(1-4)) and C-terminal (SP(3-11)) fragments were also found to permeate the BBB from the apical side.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0196-9781
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11814631-Amino Acid Sequence,
pubmed-meshheading:11814631-Animals,
pubmed-meshheading:11814631-Biological Transport,
pubmed-meshheading:11814631-Biological Transport, Active,
pubmed-meshheading:11814631-Blood-Brain Barrier,
pubmed-meshheading:11814631-Cattle,
pubmed-meshheading:11814631-Cell Division,
pubmed-meshheading:11814631-Cells, Cultured,
pubmed-meshheading:11814631-Cyclodextrins,
pubmed-meshheading:11814631-Electrophoresis, Capillary,
pubmed-meshheading:11814631-Endocytosis,
pubmed-meshheading:11814631-Endothelium, Vascular,
pubmed-meshheading:11814631-Hydrogen-Ion Concentration,
pubmed-meshheading:11814631-Indicators and Reagents,
pubmed-meshheading:11814631-Microscopy, Fluorescence,
pubmed-meshheading:11814631-Models, Chemical,
pubmed-meshheading:11814631-Molecular Sequence Data,
pubmed-meshheading:11814631-Naphthalenes,
pubmed-meshheading:11814631-Protein Structure, Tertiary,
pubmed-meshheading:11814631-Substance P,
pubmed-meshheading:11814631-Temperature,
pubmed-meshheading:11814631-Time Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Investigation of substance P transport across the blood-brain barrier.
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pubmed:affiliation |
Department of Pharmaceutical Chemistry, The University of Kansas, School of Pharmacy, Lawrence KS 66047, USA. slunte@ku.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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