Source:http://linkedlifedata.com/resource/pubmed/id/11814344
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2002-1-29
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| pubmed:abstractText |
The human equilibrative nucleoside transporter, hENT1, which is sensitive to inhibition by nitrobenzylthioinosine (NBMPR), is expressed in a wide variety of tissues. hENT1 is involved in the uptake of natural nucleosides, including regulation of the physiological effects of extracellular adenosine, and transports nucleoside drugs used in the treatment of cancer and viral diseases. Structure-function studies have revealed that transmembrane domains (TMD) 3 through 6 of hENT1 may be involved in binding of nucleosides. We have hypothesized that amino acid residues within TMD 3-6, which are conserved across equilibrative transporter sequences from several species, may have a critical role in the binding and transport of nucleosides. Therefore, we explored the role of point mutations of two conserved glycine residues, at positions 179 and 184 located in transmembrane domain 5 (TMD 5), using a GFP-tagged hENT1 in a yeast nucleoside transporter assay system. Mutations of glycine 179 to leucine, cysteine, or valine abolished transporter activity without affecting the targeting of the transporter to the plasma membrane, whereas more conservative mutations such as glycine to alanine or serine preserved both targeting to the plasma membrane and transport activity. Similar point mutations at glycine 184 resulted in poor targeting of hENT1 to the plasma membrane and little or no detectable functional activity. Uridine transport by G179A mutant was significantly lower (p < 0.05) and less sensitive (p < 0.05) to inhibition by NBMPR when compared to the wild-type transporter (IC(50) 7.7 +/- 0.8 nM versus 46 +/- 14.6 nM). Based on these data, we conclude that when hENT1 is expressed in yeast, glycine 179 is critical not only to the ability of hENT1 to transport uridine but also as a determinant of hENT1 sensitivity to NBMPR. In contrast, glycine 184 is likely important in targeting the transporter to the plasma membrane. This is the first identification and characterization of a critical amino acid residue of hENT1 that is important in both nucleoside transporter function and sensitivity to inhibition by NBMPR.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Equilibrative Nucleoside...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine,
http://linkedlifedata.com/resource/pubmed/chemical/Inosine,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Modulators,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SLC29A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Thioinosine,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine,
http://linkedlifedata.com/resource/pubmed/chemical/nitrobenzylthioinosine...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-2960
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
5
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| pubmed:volume |
41
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1512-9
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11814344-Alanine,
pubmed-meshheading:11814344-Animals,
pubmed-meshheading:11814344-Biological Transport,
pubmed-meshheading:11814344-Cell Line,
pubmed-meshheading:11814344-Conserved Sequence,
pubmed-meshheading:11814344-Dogs,
pubmed-meshheading:11814344-Equilibrative Nucleoside Transporter 1,
pubmed-meshheading:11814344-Genetic Vectors,
pubmed-meshheading:11814344-Glycine,
pubmed-meshheading:11814344-Guanosine,
pubmed-meshheading:11814344-Humans,
pubmed-meshheading:11814344-Inosine,
pubmed-meshheading:11814344-Membrane Transport Modulators,
pubmed-meshheading:11814344-Membrane Transport Proteins,
pubmed-meshheading:11814344-Mutagenesis, Site-Directed,
pubmed-meshheading:11814344-Point Mutation,
pubmed-meshheading:11814344-Saccharomyces cerevisiae,
pubmed-meshheading:11814344-Thioinosine,
pubmed-meshheading:11814344-Thionucleotides,
pubmed-meshheading:11814344-Transfection,
pubmed-meshheading:11814344-Uridine
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| pubmed:year |
2002
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| pubmed:articleTitle |
A single glycine mutation in the equilibrative nucleoside transporter gene, hENT1, alters nucleoside transport activity and sensitivity to nitrobenzylthioinosine.
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| pubmed:affiliation |
Department of Pharmaceutics and Department of Zoology, University of Washington, Seattle, Washington 98195, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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