Linked Life Data

11809753

Source:http://linkedlifedata.com/resource/pubmed/id/11809753

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2002-3-25
pubmed:abstractText
Although peroxisome proliferator-activated receptor (PPAR) delta is widely expressed in many tissues, the role of PPARdelta is poorly understood. In this study, we report that PPARdelta was up-regulated in vascular smooth muscle cells (VSMC) during vascular lesion formation. By using Northern blot analysis, we demonstrated that PPARdelta was increased by 3-4-fold in VSMC treated with platelet-derived growth factor (PDGF) (20 ng/ml). In addition, PDGF-induced PPARdelta mRNA expression neither needs de novo protein synthesis nor affects the stability of PPARdelta mRNA in VSMC. Preincubation of VSMC with phosphatidylinositol 3-kinase inhibitor (LY294002, 50 micromol/liter) or infection of VSMC with an adenovirus carrying the gene for a dominant negative form of Akt abrogated PDGF-induced PPARdelta mRNA expression, suggesting that phosphatidylinositol 3-kinase/Akt signaling pathway is involved in the regulation of PDGF-induced PPARdelta mRNA expression in VSMC. To explore the role of PPARdelta in VSMC, we generated rat vascular smooth muscle cells (A7r5) stably overexpressing PPARdelta and the control green fluorescent protein. Overexpression of PPARdelta in VSMC increased post-confluent cell proliferation by increasing the cyclin A and CDK2 as well as decreasing p57(kip2). Taken together, the results suggest that PPARdelta plays an important role in the pathology of diseases associated with VSMC proliferation, such as primary atherosclerosis and restenosis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11505-12
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11809753-Animals, pubmed-meshheading:11809753-Cell Division, pubmed-meshheading:11809753-Cells, Cultured, pubmed-meshheading:11809753-Chromones, pubmed-meshheading:11809753-Enzyme Inhibitors, pubmed-meshheading:11809753-Male, pubmed-meshheading:11809753-Morpholines, pubmed-meshheading:11809753-Muscle, Smooth, Vascular, pubmed-meshheading:11809753-Phosphatidylinositol 3-Kinases, pubmed-meshheading:11809753-Platelet-Derived Growth Factor, pubmed-meshheading:11809753-RNA, Messenger, pubmed-meshheading:11809753-Rats, pubmed-meshheading:11809753-Rats, Sprague-Dawley, pubmed-meshheading:11809753-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:11809753-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11809753-Signal Transduction, pubmed-meshheading:11809753-Transcription Factors, pubmed-meshheading:11809753-Up-Regulation
pubmed:year
2002
pubmed:articleTitle
Peroxisome proliferator-activated receptor delta is up-regulated during vascular lesion formation and promotes post-confluent cell proliferation in vascular smooth muscle cells.
pubmed:affiliation
Cardiovascular Research Institute, Morehouse School of Medicine, Atlanta, Georgia 30310.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't