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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-1-25
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pubmed:abstractText |
Melanoma cells are relatively resistant to adenovirus vector (Ad)-mediated gene transfer due to the low expression of Coxsackie-adenovirus receptor (CAR), which acts as a primitive Ad-receptor. Therefore, extremely high doses of Ad are required for effective gene therapy against melanoma. In the present study, we investigated whether fiber-mutant Ad containing the Arg-Gly-Asp (RGD) sequence in the fiber knob could promote gene delivery and anti-tumor effects in the murine B16 BL6 tumor model. B16 BL6 cells (in vitro) and tumors (in vivo) infected with RGD fiber-mutant Ad containing a tumor necrosis factor alpha gene (Ad-RGD-TNFalpha) produced more TNFalpha than those infected with conventional Ad-TNFalpha. In addition, Ad-RGD-TNFalpha required about one-tenth the dosage of Ad-TNFalpha for induction of equal therapeutic effects upon intratumoral injection into established B16 BL6 tumors. Furthermore, the combination of both TNFalpha- and interleukin 12-expressing RGD fiber-mutant Ads exhibited more effective tumor regression than the Ad expressing each alone. These results suggested that the fiber-mutant for altering Ad-tropism is a very potent technology for advancing gene therapy for melanoma.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/arginyl-glycyl-aspartic acid,
http://linkedlifedata.com/resource/pubmed/chemical/hexon capsid protein, Adenovirus
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
57-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11809531-Adenoviridae,
pubmed-meshheading:11809531-Animals,
pubmed-meshheading:11809531-Antigens, Viral,
pubmed-meshheading:11809531-Antineoplastic Agents,
pubmed-meshheading:11809531-Capsid,
pubmed-meshheading:11809531-Capsid Proteins,
pubmed-meshheading:11809531-Drug Delivery Systems,
pubmed-meshheading:11809531-Female,
pubmed-meshheading:11809531-Gene Therapy,
pubmed-meshheading:11809531-Gene Transfer Techniques,
pubmed-meshheading:11809531-Genetic Vectors,
pubmed-meshheading:11809531-Humans,
pubmed-meshheading:11809531-Luciferases,
pubmed-meshheading:11809531-Melanoma, Experimental,
pubmed-meshheading:11809531-Mice,
pubmed-meshheading:11809531-Mice, Inbred C57BL,
pubmed-meshheading:11809531-Mutation,
pubmed-meshheading:11809531-Oligopeptides,
pubmed-meshheading:11809531-Receptors, Virus,
pubmed-meshheading:11809531-Skin Neoplasms,
pubmed-meshheading:11809531-Tumor Cells, Cultured,
pubmed-meshheading:11809531-Tumor Necrosis Factor-alpha
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pubmed:year |
2002
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pubmed:articleTitle |
Fiber-mutant technique can augment gene transduction efficacy and anti-tumor effects against established murine melanoma by cytokine-gene therapy using adenovirus vectors.
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pubmed:affiliation |
Department of Pharmaceutics, School of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Kyuban-cho, Koshien, Nishinomiya, Hyogo 663-8179, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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