Source:http://linkedlifedata.com/resource/pubmed/id/11804968
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2002-1-23
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| pubmed:databankReference | |
| pubmed:abstractText |
The Musashi1 (Msi1) gene identified in mouse is a member of a subfamily of RNA binding proteins that are highly conserved across species. Msi1 expression is highly enriched in proliferative cells within the developing central nervous system. Within the testis, proliferation and differentiation of germ cells takes place within the seminiferous epithelium, where these cells are supported physically and functionally by Sertoli cells that do not themselves proliferate following the onset of puberty. RNA binding proteins expressed in testicular germ cells are essential for normal fertility. Preliminary data suggested the mRNA for Msi1 was present in ovary; therefore, we used an Msi1-specific cRNA and monoclonal antibody to investigate whether Msi1 was expressed in the testis. Msi1 mRNA was expressed in rat testis from birth until adulthood; in situ hybridization revealed silver grains within the seminiferous epithelium. Immunohistochemical studies demonstrated that at all ages examined (from Fetal Day 14.5 until adulthood) Msi1 protein was expressed in Sertoli cells. In fetal and adult rat ovaries, Msi1 was detected in granulosa cells and their precursors. In Sertoli cells, protein was detected in both cytoplasmic and nuclear compartments; in adult testes, the immunointensity of the nuclear staining was stage dependent, with highest levels of expression in Sertoli cells at stages I-VI. In rat gonads, the RNA binding protein Msi1 is expressed in both proliferating and nonproliferating Sertoli and granulosa cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ethidium,
http://linkedlifedata.com/resource/pubmed/chemical/Msi1h protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vimentin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
66
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
500-7
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| pubmed:dateRevised |
2008-9-17
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| pubmed:meshHeading |
pubmed-meshheading:11804968-Aging,
pubmed-meshheading:11804968-Animals,
pubmed-meshheading:11804968-Blotting, Northern,
pubmed-meshheading:11804968-Cell Nucleus,
pubmed-meshheading:11804968-Cytoplasm,
pubmed-meshheading:11804968-Ethidium,
pubmed-meshheading:11804968-Female,
pubmed-meshheading:11804968-Fetus,
pubmed-meshheading:11804968-Fluorescent Antibody Technique,
pubmed-meshheading:11804968-Germ Cells,
pubmed-meshheading:11804968-Immunohistochemistry,
pubmed-meshheading:11804968-In Situ Hybridization,
pubmed-meshheading:11804968-Male,
pubmed-meshheading:11804968-Molecular Sequence Data,
pubmed-meshheading:11804968-Nerve Tissue Proteins,
pubmed-meshheading:11804968-Ovarian Follicle,
pubmed-meshheading:11804968-RNA, Messenger,
pubmed-meshheading:11804968-RNA-Binding Proteins,
pubmed-meshheading:11804968-Rats,
pubmed-meshheading:11804968-Seminiferous Tubules,
pubmed-meshheading:11804968-Sertoli Cells,
pubmed-meshheading:11804968-Testis,
pubmed-meshheading:11804968-Vimentin
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| pubmed:year |
2002
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| pubmed:articleTitle |
RNA binding protein Musashi1 is expressed in sertoli cells in the rat testis from fetal life to adulthood.
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| pubmed:affiliation |
MRC Human Reproductive Sciences Unit, Edinburgh EH3 9ET, United Kingdom. p.aunders@ed.ac.uk
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| pubmed:publicationType |
Journal Article
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