Linked Life Data

11804857

Source:http://linkedlifedata.com/resource/pubmed/id/11804857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-1-23
pubmed:abstractText
5' mRNA variants of neuronal nitric oxide synthase (nNOS) are generated either by alternative promoter usage resulting in different mRNAs that encode for the same protein (nNOSalpha) or alternative splicing encoding NH(2)-terminally truncated proteins (nNOSbeta/gamma) that lack the PDZ/GLGF domain for protein-protein interaction of nNOSalpha. We studied the expression of 5' nNOS mRNA forms and nNOS-interacting proteins (postsynaptic density protein-95; PSD-95) in the rat gastrointestinal tract and analyzed the more distinct localization of nNOS protein variants in the duodenum by immunohistochemistry with COOH- and NH(2)-terminal nNOS antibodies. 5' nNOS mRNA variants showed a site-specific expression along the gastrointestinal tract with presence of all forms (nNOSalpha-a, -b, -c; nNOSbeta) in the muscle layer of esophagus, stomach, duodenum, longitudinal muscle layer of jejunum/ileum, proximal colon, and rectum. In contrast, a lack of nNOSalpha-a and nNOSbeta mRNA was observed in pylorus, circular muscle layer of jejunum/ileum, and cecum. Expression of nNOSalpha and nNOSbeta cDNAs revealed proteins of ~155 kDa and 135/125 kDa, respectively. Immunohistochemistry showed a differential distribution of COOH- and NH(2)-terminal nNOS immunoreactivity in distinct layers of rat duodenum, suggesting a cell-specific expression and distinct compartmentalization of nNOS proteins. Observed distribution of 5' nNOS mRNA variants and proteins argue for a complex control of nNOS expression by usage of separate promoters, cell- and site-specific splicing mechanisms, and translational initiation. These mechanisms could be involved in gastrointestinal motor diseases and may explain the phenotype of nNOSalpha knockout mice with gastric stasis and pyloric stenosis, due to a total loss of nNOS in the pyloric sphincter region.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0193-1857
pubmed:author
pubmed:issnType
Print
pubmed:volume
282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G349-58
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11804857-Alternative Splicing, pubmed-meshheading:11804857-Animals, pubmed-meshheading:11804857-Blotting, Western, pubmed-meshheading:11804857-COS Cells, pubmed-meshheading:11804857-Colon, pubmed-meshheading:11804857-DNA, Complementary, pubmed-meshheading:11804857-Digestive System, pubmed-meshheading:11804857-Duodenum, pubmed-meshheading:11804857-Enteric Nervous System, pubmed-meshheading:11804857-Esophagus, pubmed-meshheading:11804857-Gene Expression Regulation, Enzymologic, pubmed-meshheading:11804857-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:11804857-Male, pubmed-meshheading:11804857-Membrane Proteins, pubmed-meshheading:11804857-Nerve Tissue Proteins, pubmed-meshheading:11804857-Nitric Oxide Synthase, pubmed-meshheading:11804857-Nitric Oxide Synthase Type I, pubmed-meshheading:11804857-Organ Specificity, pubmed-meshheading:11804857-Promoter Regions, Genetic, pubmed-meshheading:11804857-Pylorus, pubmed-meshheading:11804857-RNA, Messenger, pubmed-meshheading:11804857-RNA Processing, Post-Transcriptional, pubmed-meshheading:11804857-Rats, pubmed-meshheading:11804857-Rats, Wistar, pubmed-meshheading:11804857-Stomach, pubmed-meshheading:11804857-Synapses, pubmed-meshheading:11804857-Transcription, Genetic
pubmed:year
2002
pubmed:articleTitle
Site-specific gene expression of nNOS variants in distinct functional regions of rat gastrointestinal tract.
pubmed:affiliation
Department of Internal Medicine II, Technical University of Munich, Ismaningerstrasse 22, 81675 Munich, Germany. Dieter.Saur@lrz.tu-muenchen.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't