Linked Life Data

11804041

Source:http://linkedlifedata.com/resource/pubmed/id/11804041

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-1-23
pubmed:abstractText
Liquid chromatography with an electrospray interface to a mass spectrometer (LC-ES-MS) and LC-ES coupled to a tandem MS (LC-ES-MS/MS) were used to detect and identify intermediates excreted transiently during the aerobic degradation of linear alkylbenzenesulfonates (LAS) in fixed-bed bioreactors (FBBR). The inoculum for the FBBR was the microflora of the River Rhine, Germany. Two major phenomena were observed on the addition of 100 mg/L LAS to the system, sorption and then biodegradation. Disappearance due to sorption was followed in an inhibited FBBR. Biodegradation of LAS started on day 7 and was accompanied by the transient excretion of intermediates, which were later largely degraded. We detected not only the sulfophenylcarboxylates (SPCs) observed previously but also the alpha,beta-unsaturated SPCs (SPC-2H), which have not been reported before. Experiments with the (4-sulfophenyl)dodecanes (C12-LAS), which had minor contaminants of C11-LAS, showed C12-, C10-, C8-, C6-, and C4-SPCs when LAS was degraded as well as traces of C9-, C7-, and C5-SPCs. Signals from the SPC-2H species were usually some 10% of those from the corresponding SPCs. Samples from these experiments were also examined by gas chromatography-mass spectrometry (GC-MS), but no desulfonated intermediates were detected. We interpret the data to mean that the only attack on LAS was by (omega-oxygenation; there was no visible initial desulfonation. The products of omega-oxygenation were oxidized to the corresponding SPC and subject to beta-oxidation, as evidenced not only by the pattern of C-2 units in the excreted SPCs but also in the corresponding series of SPC-2H, representing the intermediates in beta-oxidation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0730-7268
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Alpha,beta-unsaturated sulfophenylcarboxylates as degradation intermediates of linear alkylbenzenesulfonates: evidence for omega-oxygenation followed by beta-oxidations by liquid chromatography-mass spectrometry.
pubmed:affiliation
ESWE-Institute for Water Research and Water Technology, Wiesbaden, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't