Linked Life Data

11803553

Source:http://linkedlifedata.com/resource/pubmed/id/11803553

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-1-22
pubmed:abstractText
This study investigated the possibility of producing rabbits from embryos obtained by transfer of somatic cell nuclei; Muscle biopsies were taken from the upper part of the hind limb of fetuses at 24 days of gestation. Fetal fibroblasts were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FCS at 38 degrees C in 5% CO(2) in air. Cells were passaged at least three times; for 5 days prior to nuclear transfer, cells were cultured in 0.5% FCS medium to induce them into G(0). These cells then were placed into the perivitelline space of enucleated rabbit MII stage oocytes. Fusion media were Ca/Mg-containing 0.3 M mannitol (Buffer 1) or 0.3 M mannitol plus 7.5 microg/ml cytochalasin B (CB) (Buffer 2). Fusion was studied with 6 protocols: culture of donor cell-cytoplast complexes (CCCs) in TCM199 for 0, 30, or 60 min after micromaniupulation and electrical stimulation in Buffer 1 or Buffer 2. Fusion rates were 57.4, 42.9 and 52.8%, respectively, in groups 0 + B1, 30 + B1, and 60 + B1, significantly lower than the rates in groups 0 + B2, 30 + B2 and 60 + B2 (75.9, 75.7, and 76.4%, respectively) (P < 0.05). Thus, CB in electrical fusion buffer improved the fusion rate. The percentages of blastocyst formation were 40% in 0 + B2 and 37.1% in 0 + B1 groups, significantly higher (P < 0.05) than those in 30 + B1 (20%), 30 + B2 (15.6%), 60 + B1 (13%) and 60 + B2 (8.3%). A total of 653 cloned embryos were at 1-cell, 2-4-cell, or morula/blastocyst stages were transferred to 44 mated or non-mated synchronized recipients. No cloned embryos developed to term.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1040-452X
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
187-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Rabbit cloning: improved fusion rates using cytochalasin B in the fusion buffer.
pubmed:affiliation
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, P. R. China. guangpengli@yahoo.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't