|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2002-1-22
|
|
pubmed:abstractText |
The effect of 17beta-estradiol on intracellular Ca(2+) concentrations ([Ca(2+)](i)) in Madin Darby canine kidney cells was investigated by using the fluorescent dye fura-2. 17Beta-estradiol (5-100 micromol/l) induced instantaneous increases in [Ca(2+)](i) in a concentration-dependent manner. Ca(2+) removal inhibited 45 +/- 15% of the Ca(2+) signal. In Ca(2+)-free medium, pretreatment with 50 micromol/l 17beta-estradiol abolished the [Ca(2+)](i) increases induced by 2 micromol/l carbonylcyanide m-chlorophenylhydrazone (CCCP; a mitochondrial uncoupler), 1 micromol/l thapsigargin (an endoplasmic reticulum Ca(2+) pump inhibitor) and 50 micromol/l brefeldin A (an antibiotic which disperses the Golgi complex), but pretreatment with brefeldin A, CCCP and thapsigargin only partly inhibited the 17beta-estradiol-induced [Ca(2+)](i) signal. Adding 3 mmol/l Ca(2+) increased [Ca(2+)](i) in cells pretreated with 5-100 micromol/l 17beta-estradiol in Ca(2+)-free medium. Pretreatment with 1 micromol/l U73122 to abolish the formation of inositol-1,4,5-trisphosphate inhibited 50% of the Ca(2+) release induced by 50 micromol/l 17beta-estradiol. 17Beta-estradiol (20 micromol/l) also increased [Ca(2+)](i) in human bladder cancer cells and prostate cancer cells. Collectively, this study shows that 17beta-estradiol evoked a significant internal Ca(2+) release and external Ca(2+) entry possibly in a nongenomic manner.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Brefeldin A,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Macrolides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Uncoupling Agents
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0031-7012
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2002 S. Karger AG, Basel
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
64
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
84-90
|
|
pubmed:dateRevised |
2005-11-3
|
|
pubmed:meshHeading |
pubmed-meshheading:11803248-Animals,
pubmed-meshheading:11803248-Anti-Bacterial Agents,
pubmed-meshheading:11803248-Brefeldin A,
pubmed-meshheading:11803248-Calcium,
pubmed-meshheading:11803248-Calcium Signaling,
pubmed-meshheading:11803248-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:11803248-Cell Line,
pubmed-meshheading:11803248-Dose-Response Relationship, Drug,
pubmed-meshheading:11803248-Enzyme Inhibitors,
pubmed-meshheading:11803248-Estradiol,
pubmed-meshheading:11803248-Estrenes,
pubmed-meshheading:11803248-Humans,
pubmed-meshheading:11803248-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:11803248-Ion Transport,
pubmed-meshheading:11803248-Ionophores,
pubmed-meshheading:11803248-Kidney Tubules,
pubmed-meshheading:11803248-Macrolides,
pubmed-meshheading:11803248-Male,
pubmed-meshheading:11803248-Pyrrolidinones,
pubmed-meshheading:11803248-Thapsigargin,
pubmed-meshheading:11803248-Tumor Cells, Cultured,
pubmed-meshheading:11803248-Uncoupling Agents
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Effect of 17beta-estradiol on intracellular Ca(2+) levels in renal tubular cells.
|
|
pubmed:affiliation |
Division of Urology, Ping Tung Christian Hospital, Ping Tung, Taiwan.
|
|
pubmed:publicationType |
Journal Article
|
