| pubmed-article:11798085 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11798085 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:11798085 | lifeskim:mentions | umls-concept:C0021069 | lld:lifeskim |
| pubmed-article:11798085 | lifeskim:mentions | umls-concept:C1704241 | lld:lifeskim |
| pubmed-article:11798085 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:11798085 | lifeskim:mentions | umls-concept:C1171824 | lld:lifeskim |
| pubmed-article:11798085 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:11798085 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:11798085 | pubmed:dateCreated | 2002-1-18 | lld:pubmed |
| pubmed-article:11798085 | pubmed:abstractText | Immunoprecipitation with anti-human immunoglobulin G-agarose was evaluated for the detection of prolactin-immunoglobulin G (PRL-IgG) complexes in macroprolactinemic samples from hyperprolactinemic patients (prolactin measured using the automated Elecsys PRL assay; Roche Diagnostics, Mannheim, Germany). Using the polyethylene glycol (PEG) precipitation test on serum samples with PRL above 1000 mIU/l, we detected macroprolactin in 38 out of 175 samples with a recovery below 50%. Gel filtration chromatography on a subset of hyperprolactinemic samples confirmed that macroprolactin was the predominant immunoreactive form of PRL in samples which showed a recovery < or = 50%. In 37 out of 38 samples containing macroprolactin, immunoprecipitation with anti-human IgG (anti-hIgG)-agarose revealed a higher PRL-binding (19.1-91.3%) than in the sera containing monomeric PRL (< 10%). In conclusion, we found that PEG precipitation detected macroprolactin in 21.7% of samples with elevated PRL levels as measured by the Elecsys PRL assay, and that in the vast majority of these samples the presence of PRL-IgG complexes could be demonstrated by immunoprecipitation with anti-hIgG-agarose. In view of the high prevalence of PRL-IgG complexes in hyperprolactinemic patients and the simplicity of the test, immunoprecipitation with anti-hIgG-agarose might play a role in the routine laboratory handling of hyperprolactinemic samples, especially with regards to PRL assays where PEG causes interference. | lld:pubmed |
| pubmed-article:11798085 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11798085 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11798085 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11798085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11798085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11798085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11798085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11798085 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11798085 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11798085 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11798085 | pubmed:issn | 1434-6621 | lld:pubmed |
| pubmed-article:11798085 | pubmed:author | pubmed-author:De SchepperJJ | lld:pubmed |
| pubmed-article:11798085 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:11798085 | pubmed:author | pubmed-author:SmitzJJ | lld:pubmed |
| pubmed-article:11798085 | pubmed:author | pubmed-author:VelkeniersBB | lld:pubmed |
| pubmed-article:11798085 | pubmed:author | pubmed-author:Schiettecatte... | lld:pubmed |
| pubmed-article:11798085 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11798085 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:11798085 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11798085 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11798085 | pubmed:pagination | 1244-8 | lld:pubmed |
| pubmed-article:11798085 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:meshHeading | pubmed-meshheading:11798085... | lld:pubmed |
| pubmed-article:11798085 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11798085 | pubmed:articleTitle | Rapid detection of macroprolactin in the form of prolactin-immunoglobulin G complexes by immunoprecipitation with anti-human IgG-agarose. | lld:pubmed |
| pubmed-article:11798085 | pubmed:affiliation | Laboratory of Radioimmunology, University Hospital, Brussels Free University (Dutch speaking), Belgium. Johan.Schiettecatte@az.vub.ac.be | lld:pubmed |
| pubmed-article:11798085 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11798085 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
