Linked Life Data

11797836

Source:http://linkedlifedata.com/resource/pubmed/id/11797836

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-1-18
pubmed:abstractText
DT40 is a chicken B lymphocyte cell line that exhibits a high ratio of targeted and random integration of transfected DNA constructs. Using the DT40 cell line makes it comparatively easy to disrupt multiple genes in a single cell and to generate conditional targeted mutants including tet-controlled cre-lox-mediated and temperature-sensitive mutants. The DT40 mutants show a strong phenotypic resemblance to murine mutants with respect to genes involved in DNA recombination and repair. Because of these characteristics, DT40 is an attractive model for the analysis of DNA recombination and repair studies in vertebrates despite obvious concerns associated with the use of a transformed cell line that may have certain cell-line-specific characteristics. We present experimental evidence to demonstrate the usefulness of the DT40 cell line as a unique model to study DNA damaging events and their associated repair pathways.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0731-8898
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
DNA repair studies: experimental evidence in support of chicken DT40 cell line as a unique model.
pubmed:affiliation
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't