Linked Life Data

11797665

Source:http://linkedlifedata.com/resource/pubmed/id/11797665

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-1-18
pubmed:abstractText
Our laboratory is undertaking tissue engineering of liver using enriched liver progenitor cells. We report here our ongoing study to design biodegradable and biocompatible three-dimensional substratum supports of both natural and synthetic polymeric materials suitable to the adhesion, growth, and differentiation of adult and progenitor liver cells for their transplantation, and for the development of a bioartificial liver assist device. Porous biocompatible and biodegradable microcarriers of diameter 20-40 microm and 100-300 microm were prepared from (alpha-hydroxy) acid family of polymers. Human hepatoma cell line HepG2 and adult rodent liver cells were found to attach to collagen-coated surface of poly(D,L-lactide-co-glycotide) microcarriers. HepG2 cells attached to the degradable microcarriers remained viable and underwent growth expansion, forming three-dimensional cell-degradable microcarrier colonies in culture. These cell-degradable microcarrier colonies may undergo further growth expansion, thus providing a viable approach for three-dimensional organogenesis of tissue.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:volume
944
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
144-59
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Soft, porous poly(D,L-lactide-co-glycotide) microcarriers designed for ex vivo studies and for transplantation of adherent cell types including progenitors.
pubmed:affiliation
Department of Cell & Molecular Physiology, School of Medicine, University of North Carolina, Chapel Hill 27599-7038, USA. axu@med.unc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't