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rdf:type |
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|
lifeskim:mentions |
|
|
pubmed:issue |
5
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|
pubmed:dateCreated |
2002-1-18
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|
pubmed:abstractText |
The fusion gene of BPI23 and human Fc gamma 1 was obtained by PCR method, and the expression plasmid was constructed to express recombinant BPI23-Fc gamma 1 fusion protein in CHO cells. After transfection with the plasmid and selection by methotrexate, the cell lines expressing the fusion protein were obtained. The recombinant protein was purified using cation-exchange chromatography and its bioactivity was proved with bactericidal assays.
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pubmed:language |
chi
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
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|
pubmed:issn |
1000-3061
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
17
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|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
587-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11797228-Amino Acid Sequence,
pubmed-meshheading:11797228-Animals,
pubmed-meshheading:11797228-Antimicrobial Cationic Peptides,
pubmed-meshheading:11797228-Blood Proteins,
pubmed-meshheading:11797228-CHO Cells,
pubmed-meshheading:11797228-Cricetinae,
pubmed-meshheading:11797228-Dose-Response Relationship, Drug,
pubmed-meshheading:11797228-Escherichia coli,
pubmed-meshheading:11797228-Gene Expression,
pubmed-meshheading:11797228-Humans,
pubmed-meshheading:11797228-Membrane Proteins,
pubmed-meshheading:11797228-Molecular Sequence Data,
pubmed-meshheading:11797228-Plasmids,
pubmed-meshheading:11797228-Receptors, IgG,
pubmed-meshheading:11797228-Recombinant Fusion Proteins,
pubmed-meshheading:11797228-Time Factors,
pubmed-meshheading:11797228-Transfection
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|
pubmed:year |
2001
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|
pubmed:articleTitle |
[Expression of recombinant BPI23-Fc gamma 1 fusion protein in CHO cells].
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|
pubmed:affiliation |
Institute of Microbiology and Epidemiology, Beijing 100071, China. xujunjie@sina.com
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pubmed:publicationType |
Journal Article,
English Abstract
|
