Linked Life Data

11795393

Source:http://linkedlifedata.com/resource/pubmed/id/11795393

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-1-17
pubmed:abstractText
In order to assist in the identification of possible endocrine disrupting chemicals (EDC) in groundwater, we are developing Caenorhabolitis elegans as a high throughput bioassay system in which responses to EDC may be detected by gene expression using DNA microarray analysis. As a first step we examined gene expression patterns and vitellogenin responses of this organism to vertebrate steroids, in liquid culture. Western blotting showed the expected number and size of vitellogenin translation products after estrogen exposure. At 10(-9) M, vitellogenin decreased, but at 10(-7) and 10(-5), vitellogenin was increased. Testosterone (10(-5) M) increased the synthesis of vitellogenin, but progesterone-treated cultures (10(-5) M) had less vitellogenin. Using DNA microarray analysis, we examined the pattern of gene expression after progesterone (10(-5), 10(-7), and 10(-9) M), estrogen (10(-5) M), and testosterone (10(-9) M) exposure, with special attention to the traditional biomarker genes used in environmental studies [vitellogenin, cytochrome P450 (CYP), glutathione s-transferase (GST), metallothionein (MT), and heat shock proteins (HSP)]. GST and P450 genes were affected by estrogen (10(-5) M) and progesterone (10(-5) and 10(-7) M) treatments. For vitellogenin genes, estrogen treatment (10(-5) M) caused overexpression of the vit-2 and vit-6 genes (2.68 and 3.25 times, respectively). After progesterone treatment (10(-7) M), the vit-5 and vit-6 were down-regulated and vit-1 up-regulated (3.59-fold). Concentrations of testosterone and progesterone at 10(-9) M did not influence the expression of the vit, CYP, or GST genes. Although the analysis is incomplete, and low doses and combinations of EDC need to be tested, these preliminary results indicate C. elegans may be a useful laboratory and field model for screening EDC.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:volume
948
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11795393-Animals, pubmed-meshheading:11795393-Blotting, Western, pubmed-meshheading:11795393-Caenorhabditis elegans, pubmed-meshheading:11795393-Cholesterol, pubmed-meshheading:11795393-Egg Proteins, pubmed-meshheading:11795393-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:11795393-Environmental Monitoring, pubmed-meshheading:11795393-Female, pubmed-meshheading:11795393-Gene Expression Profiling, pubmed-meshheading:11795393-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:11795393-Progesterone, pubmed-meshheading:11795393-RNA, Messenger, pubmed-meshheading:11795393-Steroids, pubmed-meshheading:11795393-Testosterone, pubmed-meshheading:11795393-Transcription, Genetic, pubmed-meshheading:11795393-Vitellogenins, pubmed-meshheading:11795393-Water Pollutants, Chemical
pubmed:year
2001
pubmed:articleTitle
Caenorhabditis elegans as an environmental monitor using DNA microarray analysis.
pubmed:affiliation
Department of Biology, Boston University, Massachusetts 02215, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't