Source:http://linkedlifedata.com/resource/pubmed/id/11792376
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rdf:type | |
lifeskim:mentions |
umls-concept:C0014441,
umls-concept:C0021758,
umls-concept:C0086418,
umls-concept:C0205314,
umls-concept:C0205359,
umls-concept:C0599161,
umls-concept:C0679622,
umls-concept:C0871261,
umls-concept:C0920508,
umls-concept:C1511790,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1707455,
umls-concept:C2911692
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pubmed:issue |
1-2
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pubmed:dateCreated |
2002-1-16
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pubmed:abstractText |
Interleukin-4 (IL-4) is an important T-helper cell type 2 (Th2) cytokine in man, driving Th2 polarisation and exerting the most antagonistic effects to the Th1 cytokine interferon-gamma (IFN-gamma). Nevertheless, few data on spontaneous and antigen-specific secretion of IL-4 in man are available, mainly due to difficulties in the detection of IL-4. In this study, we compared three assays that can detect antigen-induced IL-4 responses; ELISPOT, ELISA after blocking the IL-4 receptor during cell culture, and real-time reverse transcription polymerase chain reaction (RT-PCR). Spontaneous, antigen- and allergen-induced responses were analysed in peripheral blood mononuclear cells from three groups with different secretion patterns for IL-4: atopic individuals, nonatopic individuals and pregnant women. ELISPOT displayed the highest sensitivity and was the only assay that could detect spontaneous secretion of IL-4 in all analysed samples. The IL-4 receptor blocking ELISA was considered best for the detection of in vitro antigen- and allergen-induced responses, since the results obtained from the ELISPOT and real-time RT-PCR displayed lower specificity, possibly because of seemingly aberrant IL-4 responses in the group of pregnant women. The real-time RT-PCR for detection of IL-4 mRNA proved to be sensitive, but expression of IL-4 mRNA was not correlated with the secretion of IL-4.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
260
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-67
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11792376-Adult,
pubmed-meshheading:11792376-Cells, Cultured,
pubmed-meshheading:11792376-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11792376-Female,
pubmed-meshheading:11792376-Humans,
pubmed-meshheading:11792376-Hypersensitivity, Immediate,
pubmed-meshheading:11792376-Interleukin-4,
pubmed-meshheading:11792376-Male,
pubmed-meshheading:11792376-Middle Aged,
pubmed-meshheading:11792376-Pregnancy,
pubmed-meshheading:11792376-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11792376-Th1 Cells,
pubmed-meshheading:11792376-Th2 Cells
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pubmed:year |
2002
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pubmed:articleTitle |
Detection of spontaneous and antigen-induced human interleukin-4 responses in vitro: comparison of ELISPOT, a novel ELISA and real-time RT-PCR.
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pubmed:affiliation |
Clinical Research Centre, Faculty of Health Sciences, University Hospital, S-581 85, Linköping, Sweden. Christina.Ekerfelt@kfc.liu.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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