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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-1-15
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pubmed:abstractText |
Among cytomegalovirus (CMV) tegument proteins, phosphoprotein 65 (pp65) has been identified as the important target antigen of the cytotoxic T lymphocyte (CTL) response against the virus. We synthesized seven CMV-pp65-derived peptides carrying an HLA-A24-binding motif, and investigated the ability of these peptides to induce CMV-specific CTL. We identified one nonamer peptide (pp65113-121; VYALPLKML) able to bind HLA-A24 and induce CTL responses in vitro in peripheral blood mononuclear cells (PBMC) from CMV-seropositive individuals. The peptide-specific CTLs generated were capable of recognizing pp65 expressed on CMV-infected fibroblasts as well as pp65113-121 peptide bound to the surface of C1R-A*2402 cells in an HLA-A24-restricted manner. The pp65113-121 peptide thus might be considered a synthetic peptide vaccine in HLA-A24-positive individuals.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0882-8245
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
369-77
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11792066-Antigens, Viral,
pubmed-meshheading:11792066-Cells, Cultured,
pubmed-meshheading:11792066-Cytomegalovirus,
pubmed-meshheading:11792066-Epitopes, T-Lymphocyte,
pubmed-meshheading:11792066-HLA-A Antigens,
pubmed-meshheading:11792066-HLA-A24 Antigen,
pubmed-meshheading:11792066-Humans,
pubmed-meshheading:11792066-Peptides,
pubmed-meshheading:11792066-Phosphoproteins,
pubmed-meshheading:11792066-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11792066-Viral Matrix Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Identification of the HLA-A24 peptide epitope within cytomegalovirus protein pp65 recognized by CMV-specific cytotoxic T lymphocytes.
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pubmed:affiliation |
First Department of Internal Medicine, Kurume University School of Medicine, Japan. megum@med.kurume-u.ac.jp
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pubmed:publicationType |
Journal Article
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