Linked Life Data

11791856

Source:http://linkedlifedata.com/resource/pubmed/id/11791856

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2002-1-15
pubmed:abstractText
Analysis of 56 river water samples by the Enterolert defined substrate technique, and standard m-Enterococcus agar isolation followed by confirmation, indicated that after 24 h incubation. Enterolert significantly underestimated the true numbers of enterococci. Extending Enterolert incubatioin to 36 h improved detection but also revealed false positives. These findings prompted the development of a novel confirmation medium we have termed glucosidase agar, which was prepared by dissolving Enterolert substrate in 2% (w/v) bacteriological agar. Analysis of 1,043 colonies arising on m-Enterococcus agar from 280 freshwater, marine and sewage effluent samples, demonstrated that 2-4 h incubation on glucosidase agar was a rapid and accurate means of confirming presumptive enterococci, when compared to standard confirmation procedures that take 48 h. The combination of primary isolation on m-Enterococcus agar followed by confirmation on glucosidase agar permits maximum recovery of Enteroccus whilst effectively eliminating false positives/negatives and provides a reliable alternative use of the Enterolert defined substrate technology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0043-1354
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4243-6
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Development of glucosidase agar for the confirmation of water-borne Enterococcus.
pubmed:affiliation
Microbiology Unit, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia.
pubmed:publicationType
Journal Article, Evaluation Studies