Linked Life Data

11788942

Source:http://linkedlifedata.com/resource/pubmed/id/11788942

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-1-14
pubmed:abstractText
In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was first observed in a subset of cardiomyocytes, which was localized ventrally in the fused heart tube and laterally in the unfused cardiac sheets. Together, these results show that cANF expression can be used to distinguish differentiated chamber (working) myocardium, including the peripheral ventricular conduction system, from embryonic myocardium. We conclude that differentiation of chamber myocardium takes place already at HH9 at the ventral side of the linear heart tube, possibly preceded by latero-medial signals in the unfused cardiac sheets.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0003-276X
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
266
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-102
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11788942-Animals, pubmed-meshheading:11788942-Atrial Natriuretic Factor, pubmed-meshheading:11788942-Avian Proteins, pubmed-meshheading:11788942-Chick Embryo, pubmed-meshheading:11788942-DNA, pubmed-meshheading:11788942-DNA-Binding Proteins, pubmed-meshheading:11788942-Embryonic and Fetal Development, pubmed-meshheading:11788942-Gene Expression Regulation, Developmental, pubmed-meshheading:11788942-Heart Atria, pubmed-meshheading:11788942-Heart Ventricles, pubmed-meshheading:11788942-Homeodomain Proteins, pubmed-meshheading:11788942-In Situ Hybridization, pubmed-meshheading:11788942-Mice, pubmed-meshheading:11788942-Myocardium, pubmed-meshheading:11788942-Polymerase Chain Reaction, pubmed-meshheading:11788942-Transcription, Genetic, pubmed-meshheading:11788942-Troponin I
pubmed:year
2002
pubmed:articleTitle
Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken.
pubmed:affiliation
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't