Source:http://linkedlifedata.com/resource/pubmed/id/11788899
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2002-1-14
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| pubmed:abstractText |
WNT - beta-catenin - TCF signaling pathway is activated by Xenopus wnt-8 (Xwnt-8) during Xenopus early development, and dysregulated activation of beta-catenin - TCF signaling pathway in mammalian cells leads to carcinogenesis. We have previously cloned and characterized human WNT8A, one of human orthologues of Xwnt-8. Here, we cloned and characterized human WNT8B by using bioinformatics, cDNA-PCR, and RACE. WNT8B gene of about 23-kb in size consisted of six exons, and encoded a 351-amino-acid polypeptide with the N-terminal signal peptide and two N-linked glycosylation sites. C-terminal region of WNT8B, WNT8A, WNT2, and WNT2B were longer than that of other human WNTs. Thirty-five nucleotide changes between WNT8B isolated by us and WNT8B isolated by another group resulted in Gly230Ala and Arg284Leu amino-acid substitutions. Gly230 and Arg284 of WNT8B were conserved in WNT8A. Gly230-Arg284 WNT8B allele was also identified in human genome draft sequences AL133352.10, AL359759.18, and human EST BF732616. These results indicate that the Gly230-Arg284 WNT8B cDNA isolated in this study is derived from the more common WNT8B allele. WNT8B mRNAs of 4.4- and 3.5-kb in size were weakly detected in a colorectal cancer cell line SW480, but were undetectable in any normal human tissues by using Northern blot analyses. WNT8B was significantly up-regulated in gastric cancer cell lines KATO-III (signet-ring cell carcinoma) and MKN45 (poorly differentiated adenocarcinoma), and also in 5 out of 10 cases of primary gastric cancer. WNT8B might play key roles in gastric cancer through activation of the beta-catenin - TCF signaling pathway.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/WNT8B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1019-6439
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
343-8
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11788899-Alleles,
pubmed-meshheading:11788899-Amino Acid Sequence,
pubmed-meshheading:11788899-Cloning, Molecular,
pubmed-meshheading:11788899-DNA, Complementary,
pubmed-meshheading:11788899-Exons,
pubmed-meshheading:11788899-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11788899-Humans,
pubmed-meshheading:11788899-Introns,
pubmed-meshheading:11788899-Mutation,
pubmed-meshheading:11788899-Polymerase Chain Reaction,
pubmed-meshheading:11788899-Proteins,
pubmed-meshheading:11788899-RNA, Neoplasm,
pubmed-meshheading:11788899-Sequence Homology, Amino Acid,
pubmed-meshheading:11788899-Stomach Neoplasms,
pubmed-meshheading:11788899-Tumor Cells, Cultured,
pubmed-meshheading:11788899-Up-Regulation,
pubmed-meshheading:11788899-Wnt Proteins,
pubmed-meshheading:11788899-Zebrafish Proteins
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| pubmed:year |
2002
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| pubmed:articleTitle |
Up-regulation of WNT8B mRNA in human gastric cancer.
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| pubmed:affiliation |
Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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