Linked Life Data

11788857

Source:http://linkedlifedata.com/resource/pubmed/id/11788857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-1-14
pubmed:abstractText
The effects of fish antifreeze protein AFP-I on cryopreservation of rice suspension cells by three different protocols were investigated. During the two-step method, AFP-I at 0.01 mg/ml significantly lowered the viability of both precultured and non-precultured cells. During the vitrification method, AFP-I at 0.2 mg/ml improved the viability of suboptimally thawed cells; however, much higher doses of this protein (10mg/ml) attenuated the cell viability. During rapid freezing of rice cells in the solutions with relatively high (but non-vitrifying) concentrations of cryoprotectant, AFP-I displayed protective action in the higher concentrated cryoprotectants and detrimental effect in more dilute ones. Taken together, it was concluded that, depending upon a number of factors discussed in the present paper, both positive effect and negative effect could be observed during application of AFP to cryopreservation of rice cells. The possible mechanism of this dual character was discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-2044
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
The dual effect of antifreeze protein on cryopreservation of rice (Oryza sativa l.) embryogenic suspension cells.
pubmed:affiliation
College of Life Sciences, Zhejiang University, Hangzhou 310012, China. junhuiwang@cls.zju.edu.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't