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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2002-3-18
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pubmed:abstractText |
The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an E1-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus E2 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/P55-L5 protein, Borrelia burgdorferi,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BergmannStephanS,
pubmed-author:BrandKarstenK,
pubmed-author:CurielDavid TDT,
pubmed-author:DietelManfredM,
pubmed-author:DobbelsteinMatthiasM,
pubmed-author:GansbacherBerndB,
pubmed-author:HolmPer SPS,
pubmed-author:JurchottKarstenK,
pubmed-author:LadhoffAxelA,
pubmed-author:LageHermannH,
pubmed-author:MantwillKlausK,
pubmed-author:RoyerHans-DieterHD
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10427-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11788582-Active Transport, Cell Nucleus,
pubmed-meshheading:11788582-Adenoviridae,
pubmed-meshheading:11788582-Adenovirus E2 Proteins,
pubmed-meshheading:11788582-Antigens, Bacterial,
pubmed-meshheading:11788582-Bacterial Proteins,
pubmed-meshheading:11788582-Blotting, Northern,
pubmed-meshheading:11788582-Blotting, Southern,
pubmed-meshheading:11788582-Blotting, Western,
pubmed-meshheading:11788582-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:11788582-Cell Line,
pubmed-meshheading:11788582-Cell Nucleus,
pubmed-meshheading:11788582-DNA, Complementary,
pubmed-meshheading:11788582-DNA-Binding Proteins,
pubmed-meshheading:11788582-Gene Expression Regulation,
pubmed-meshheading:11788582-HeLa Cells,
pubmed-meshheading:11788582-Humans,
pubmed-meshheading:11788582-Microscopy, Confocal,
pubmed-meshheading:11788582-Microscopy, Electron,
pubmed-meshheading:11788582-Microscopy, Fluorescence,
pubmed-meshheading:11788582-NFI Transcription Factors,
pubmed-meshheading:11788582-Nuclear Proteins,
pubmed-meshheading:11788582-Promoter Regions, Genetic,
pubmed-meshheading:11788582-Protein Binding,
pubmed-meshheading:11788582-Transcription Factors,
pubmed-meshheading:11788582-Transgenes,
pubmed-meshheading:11788582-Virus Replication,
pubmed-meshheading:11788582-Y-Box-Binding Protein 1
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pubmed:year |
2002
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pubmed:articleTitle |
YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter.
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pubmed:affiliation |
Institut für Experimentelle Onkologie und Therapieforschung, Technische Universität München, Klinikum Rechts der Isar, München 81675, Germany. per.s.holm@lrz.tum.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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