Source:http://linkedlifedata.com/resource/pubmed/id/11788470
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2002-1-14
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| pubmed:abstractText |
Monocyte/macrophage infiltration to the arterial wall is an initial step in atherosclerosis, and monocyte chemoattractant protein-1 (MCP-1) is thought to play a central role in the recruitment of these cells. In the present study, we examined the role of local expression of MCP-1 at the vessel wall in the initiation and development of atherosclerosis. We transfected the cDNA encoding rat MCP-1 into the vessel wall of the rabbit carotid artery with the use of the hemagglutinating virus of Japan (HVJ)-liposome method. The rabbits were divided into the following groups: (1) those fed normal chow and transfected with MCP-1-HVJ, (2) those fed a high cholesterol diet (1% cholesterol) and transfected with MCP-1-HVJ, and (3) those fed a high cholesterol diet and transfected with control-HVJ. Prescribed diets were started 2 weeks before transfection and were continued for another 2 weeks. In group 1, vascular lesion formation was not found, and anti-rabbit monocyte/macrophage antibody (RAM-11) staining for monocytes/macrophages was negative, although anti-rat MCP-1 antibody (R-17) staining for rat MCP-1 was positive mainly in endothelial cells. Cholesterol feeding increased plasma cholesterol levels to 1801+/-444 mg/dL in group 2. In group 2, all rabbits displayed neointimal formation with infiltration of RAM-11-positive cells, and a part of the lesion was also positive for Sudan III lipid staining. In group 3, hypercholesterolemia did not induce the infiltration of monocytes/macrophages and subsequent lesion formation in the vessel wall despite definite upregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 on the endothelium. To initiate atherosclerotic changes, local MCP-1 overexpression at the vessel is not sufficient, and activation of other factors induced by hypercholesterolemia is required.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1524-4636
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| pubmed:author |
pubmed-author:HiraseTetsuakiT,
pubmed-author:HirataKen-ichiK,
pubmed-author:InoueNobutakaN,
pubmed-author:IshidaTatsuroT,
pubmed-author:KanedaYasufumiY,
pubmed-author:KawashimaSeinosukeS,
pubmed-author:MatsukawaAkihiroA,
pubmed-author:MorishitaRyuichiR,
pubmed-author:NamikiMasayukiM,
pubmed-author:OzakiMasanoriM,
pubmed-author:YamashitaTomoyaT,
pubmed-author:YokoyamaMitsuhiroM
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| pubmed:issnType |
Electronic
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| pubmed:volume |
22
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
115-20
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11788470-Animals,
pubmed-meshheading:11788470-Antibodies, Monoclonal,
pubmed-meshheading:11788470-Arteriosclerosis,
pubmed-meshheading:11788470-Carotid Artery Diseases,
pubmed-meshheading:11788470-Cell Movement,
pubmed-meshheading:11788470-Chemokine CCL2,
pubmed-meshheading:11788470-Cholesterol,
pubmed-meshheading:11788470-Diet, Atherogenic,
pubmed-meshheading:11788470-Endothelium, Vascular,
pubmed-meshheading:11788470-Hypercholesterolemia,
pubmed-meshheading:11788470-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11788470-Macrophages,
pubmed-meshheading:11788470-Male,
pubmed-meshheading:11788470-Monocytes,
pubmed-meshheading:11788470-Rabbits,
pubmed-meshheading:11788470-Rats,
pubmed-meshheading:11788470-Transfection,
pubmed-meshheading:11788470-Up-Regulation,
pubmed-meshheading:11788470-Vascular Cell Adhesion Molecule-1
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| pubmed:year |
2002
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| pubmed:articleTitle |
Local overexpression of monocyte chemoattractant protein-1 at vessel wall induces infiltration of macrophages and formation of atherosclerotic lesion: synergism with hypercholesterolemia.
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| pubmed:affiliation |
Division of Cardiovascular and Respiratory Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
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| pubmed:publicationType |
Journal Article
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