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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-1-14
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pubmed:abstractText |
Macrophage (Mphi) prostaglandin (PG)E(2) production has been implicated in immunosuppression and increased susceptibility to sepsis after thermal injury. Deficient interleukin (IL)-12 production has also been implicated in these postburn complications. The present study examined the relationship between Mphi cyclooxygenase (COX)-2 activity and IL-12 production after thermal injury. C57BL/6 female mice were subjected to a 25% total body surface area full-thickness burn. Mphi were isolated 7 days later, or the mice were subjected to sepsis by cecal ligation and puncture (CLP). IL-12 production by Mphi from injured mice was suppressed by >50%, whereas COX-2 expression and PGE(2) production were increased twofold. The COX-2 inhibitor NS-398 suppressed PGE(2) production and normalized IL-12 production in the injury group, whereas it had no effect on IL-10 production. Injured mice subjected to CLP had lower IL-12 plasma levels compared with sham-treated mice subjected to CLP. NS-398 treatment prevented the suppression in plasma IL-12 levels in the injury group. Thus elevated Mphi COX-2 activity, independent of IL-10, suppresses Mphi IL-12 production after thermal injury and may play an important role in the observed immunosuppression under such conditions.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0363-6143
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C263-70
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11788337-Animals,
pubmed-meshheading:11788337-Bacterial Infections,
pubmed-meshheading:11788337-Burns,
pubmed-meshheading:11788337-Cyclooxygenase 2,
pubmed-meshheading:11788337-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:11788337-Cyclooxygenase Inhibitors,
pubmed-meshheading:11788337-Female,
pubmed-meshheading:11788337-Interleukin-10,
pubmed-meshheading:11788337-Interleukin-12,
pubmed-meshheading:11788337-Isoenzymes,
pubmed-meshheading:11788337-Macrophages,
pubmed-meshheading:11788337-Mice,
pubmed-meshheading:11788337-Mice, Inbred C57BL,
pubmed-meshheading:11788337-Nitrobenzenes,
pubmed-meshheading:11788337-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:11788337-Sulfonamides
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pubmed:year |
2002
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pubmed:articleTitle |
Cyclooxygenase 2-mediated suppression of macrophage interleukin-12 production after thermal injury.
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pubmed:affiliation |
Center for Surgical Research, Department of Surgery, University of Alabama at Birmingham, 35294, USA. Martin.Schwacha@ccc.uab.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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