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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1975-12-29
|
| pubmed:abstractText |
Polysomes from the mouse myeloma MOPC-21 were purified by gel filtration of Sepharose 6B, 4B and 2B columns. All three columns eliminated nearly all intracellular material smaller than 40 S subunits. In addition, passage through 4B and 2B columns substantially reduced the amount of subunits and monosomes in the preparations. Purified polysomes retained structural integrity when stored at -85 degrees C for at least nine weeks.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0032-7484
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
247-55
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1178652-Cell Fractionation,
pubmed-meshheading:1178652-Cell Line,
pubmed-meshheading:1178652-Centrifugation, Density Gradient,
pubmed-meshheading:1178652-Chromatography, Gel,
pubmed-meshheading:1178652-Freezing,
pubmed-meshheading:1178652-Polyribosomes,
pubmed-meshheading:1178652-Sepharose
|
| pubmed:year |
1975
|
| pubmed:articleTitle |
Polysome isolation of sepharose column chromatography.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|