Linked Life Data

11785985

Source:http://linkedlifedata.com/resource/pubmed/id/11785985

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-1-11
pubmed:abstractText
PACE4A is a member of the mammalian subtilisin-like proprotein convertase family which is responsible for the proteolytic activation of precursors into their biologically active forms. Previously we reported that the maturation of proPACE4A occurs via a intramolecular autoactivation and cleavage of the propeptide is a rate-limiting step for the secretion of PACE4A (Nagahama et al., FEBS Lett. (1998) 434, 155-159). Although PACE4A is a putative secretory enzyme, it matures and is secreted much slower than general secretory proteins. In this study, we investigated the molecular mechanism underlying this slow maturation. The deletion of 25 amino acids at the carboxy terminus is sufficient for a marked acceleration in both the maturation and secretion of PACE4A. The carboxyl-truncated proPACE4A existed only as a monomer-sized form in the endoplasmic reticulum, whereas the wild type of proPACE4A existed in larger forms. Further, the fusion construct of yellow fluorescent protein and the carboxy-terminal sequence of PACE4A associated with the proPACE4A moiety and inhibited maturation. Thus the carboxy terminus of PACE4A functions as a potent autoinhibitor of its activation, resulting in the retention of proPACE4A in the endoplasmic reticulum. These findings indicate that PACE4A activity is highly controlled by a unique system at post-translational level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
290
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
878-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11785985-Amino Acid Sequence, pubmed-meshheading:11785985-Cell Line, pubmed-meshheading:11785985-Enzyme Activation, pubmed-meshheading:11785985-Humans, pubmed-meshheading:11785985-Kidney, pubmed-meshheading:11785985-Molecular Sequence Data, pubmed-meshheading:11785985-Mutagenesis, Site-Directed, pubmed-meshheading:11785985-Peptide Fragments, pubmed-meshheading:11785985-Proprotein Convertases, pubmed-meshheading:11785985-Protein Precursors, pubmed-meshheading:11785985-Protein Processing, Post-Translational, pubmed-meshheading:11785985-Protein Structure, Quaternary, pubmed-meshheading:11785985-Sequence Deletion, pubmed-meshheading:11785985-Serine Endopeptidases, pubmed-meshheading:11785985-Structure-Activity Relationship, pubmed-meshheading:11785985-Subtilisins, pubmed-meshheading:11785985-Ultracentrifugation
pubmed:year
2002
pubmed:articleTitle
A critical role for the carboxy terminal region of the proprotein convertase, PACE4A, in the regulation of its autocatalytic activation coupled with secretion.
pubmed:affiliation
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minamijosanjima, Tokushima, 770-8506, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't