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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-1-8
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pubmed:abstractText |
The recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in immature lymphoid cells undergoing the recombination of antigen receptor genes. The regulation of murine RAG-2 promoter was studied and it was revealed that the -41/-17 RAG-2 promoter region, which is conserved between humans and mice, was indispensable for the RAG-2 promoter activity in B-cell lines. The region contained 2 cis elements that bound c-Myb and Pax-5. Mutation in the c-Myb-binding site in the promoter reduced the promoter activity in B-cell lines. Cooperative activation of the RAG-2 promoter was seen by a combination of c-Myb and Pax-5 in a human embryonic kidney cell line (293T), via their synergistic DNA-binding. Deletion experiments showed that the C-terminus of c-Myb was responsible for their interaction. Furthermore, the dominant-negative c-Myb mutant suppressed the activation of the RAG-2 promoter in a pre-B-cell line as well as in 293T cells. These results suggest that cooperative binding of c-Myb and Pax-5 to the RAG-2 promoter is one of the mechanisms to direct the restricted expression of the RAG-2 in immature B cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell-Specific Activator Protein,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PAX5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pax5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/RAG2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Rag2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/V(D)J recombination activating...
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
576-83
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11781241-Animals,
pubmed-meshheading:11781241-B-Cell-Specific Activator Protein,
pubmed-meshheading:11781241-B-Lymphocyte Subsets,
pubmed-meshheading:11781241-Binding Sites,
pubmed-meshheading:11781241-Cell Line,
pubmed-meshheading:11781241-DNA,
pubmed-meshheading:11781241-DNA-Binding Proteins,
pubmed-meshheading:11781241-Gene Expression Regulation,
pubmed-meshheading:11781241-Gene Targeting,
pubmed-meshheading:11781241-Genes, Dominant,
pubmed-meshheading:11781241-Genes, myb,
pubmed-meshheading:11781241-Humans,
pubmed-meshheading:11781241-Kidney,
pubmed-meshheading:11781241-Macromolecular Substances,
pubmed-meshheading:11781241-Mice,
pubmed-meshheading:11781241-Nuclear Proteins,
pubmed-meshheading:11781241-Promoter Regions, Genetic,
pubmed-meshheading:11781241-Protein Structure, Tertiary,
pubmed-meshheading:11781241-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:11781241-Recombinant Fusion Proteins,
pubmed-meshheading:11781241-Sequence Deletion,
pubmed-meshheading:11781241-Sequence Homology, Amino Acid,
pubmed-meshheading:11781241-Species Specificity,
pubmed-meshheading:11781241-Structure-Activity Relationship,
pubmed-meshheading:11781241-Transcription, Genetic,
pubmed-meshheading:11781241-Transcription Factors,
pubmed-meshheading:11781241-Transfection
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pubmed:year |
2002
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pubmed:articleTitle |
Cooperative binding of c-Myb and Pax-5 activates the RAG-2 promoter in immature B cells.
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pubmed:affiliation |
Department of Immunology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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