rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-1-7
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pubmed:abstractText |
Escherichia coli penicillin-binding protein 5 (PBP5) anchors to the inner membrane in a pH-dependent manner via a C-terminal amphiphilic alpha-helix. Low pH was found to enhance both levels of PBP5 membrane anchoring and levels of alpha-helicity in an aqueous PBP5 C-terminal homologue, which led to the suggestion that levels of PBP5 membrane anchoring are related to levels of PBP5 C-terminal alpha-helicity. Here we have used Fourier-transformed infrared spectroscopy (FTIR) and a peptide homologue of the PBP5 C-terminal sequence to investigate the effect of pH on the conformational behavior of this sequence at a lipid interface and on its ability to interact with lipid. Our results suggest that the membrane-anchoring mechanism of PBP5 is unlikely to involve conformational change in the protein's C-terminal region and may therefore involve conformational changes in the protein's ectomembranous domain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
|
pubmed:copyrightInfo |
(c)2002 Elsevier Science.
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
290
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
427-30
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:11779187-Bacterial Proteins,
pubmed-meshheading:11779187-Carrier Proteins,
pubmed-meshheading:11779187-Cell Membrane,
pubmed-meshheading:11779187-Escherichia coli,
pubmed-meshheading:11779187-Hexosyltransferases,
pubmed-meshheading:11779187-Hydrogen-Ion Concentration,
pubmed-meshheading:11779187-Lipids,
pubmed-meshheading:11779187-Muramoylpentapeptide Carboxypeptidase,
pubmed-meshheading:11779187-Penicillin-Binding Proteins,
pubmed-meshheading:11779187-Peptides,
pubmed-meshheading:11779187-Peptidyl Transferases,
pubmed-meshheading:11779187-Protein Conformation,
pubmed-meshheading:11779187-Protein Structure, Secondary,
pubmed-meshheading:11779187-Protein Structure, Tertiary,
pubmed-meshheading:11779187-Temperature
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pubmed:year |
2002
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pubmed:articleTitle |
A study on the C-terminal membrane anchoring of Escherichia coli penicillin-binding protein 5.
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pubmed:affiliation |
Division of Biophysics, Forschunginstitute Borstel, D-2061 Borstel, Germany.
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pubmed:publicationType |
Journal Article
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