Source:http://linkedlifedata.com/resource/pubmed/id/11777991
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2002-1-4
|
pubmed:abstractText |
The induction of dendritic cell (DC) maturation is critical for the induction of Ag-specific T lymphocyte responses and may be essential for the development of human vaccines relying on T cell immunity. In this study, we have investigated the effects of monophosphoryl lipid A (MPL) on human monocyte-derived DC as well as peripheral blood T cells. Calcium mobilization, mitogen-activated protein kinase activation, and the NF-kappaB transcription factor were induced after MPL stimulation of DC and required high doses of MPL (100 microg/ml). Maturation parameters such as production of IL-12 and increases in cell surface expression of HLA-DR, CD80, CD86, CD40, and CD83 were observed following DC treatment with MPL. However, lower levels of IL-12 were induced by MPL when compared with lipopolysaccharide. This is likely to be related to differences in the kinetics of extracellular signal-related kinase 1/2 and p-38 phosphorylation induced by both molecules. Although maturation induced by MPL was weaker when compared with lipopolysaccharide, it appeared to be sufficient to support optimal activation of allogeneic naive CD45RA(+) T cell and anti-tetanus toxoid CD4 T cells. MPL at low doses (5 microg/ml) had no impact on DC maturation, while its addition to DC-T cell cocultures induced full T cell activation. The observed effect was related to the fact that MPL also acts directly on T cells, likely through their Toll-like receptors, by increasing their intracellular calcium and up-regulating their CD40 ligand expression. Together, these data support a model where MPL enhances T cell responses by having an impact on DC and T cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid A,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Muromonab-CD3,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/monophosphoryl lipid A
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
168
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
926-32
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:11777991-Adjuvants, Immunologic,
pubmed-meshheading:11777991-Antigens, CD28,
pubmed-meshheading:11777991-Antigens, CD3,
pubmed-meshheading:11777991-CD40 Ligand,
pubmed-meshheading:11777991-Calcium Signaling,
pubmed-meshheading:11777991-Cell Differentiation,
pubmed-meshheading:11777991-Dendritic Cells,
pubmed-meshheading:11777991-Enzyme Activation,
pubmed-meshheading:11777991-Humans,
pubmed-meshheading:11777991-Immune Sera,
pubmed-meshheading:11777991-Lipid A,
pubmed-meshheading:11777991-Lymphocyte Activation,
pubmed-meshheading:11777991-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11777991-Monocytes,
pubmed-meshheading:11777991-Muromonab-CD3,
pubmed-meshheading:11777991-NF-kappa B,
pubmed-meshheading:11777991-T-Lymphocytes
|
pubmed:year |
2002
|
pubmed:articleTitle |
Monophosphoryl lipid A activates both human dendritic cells and T cells.
|
pubmed:affiliation |
Laboratory of Experimental Immunology, Faculté de Medecine, Universite Libre de Bruxelles, Brussels, Belgium. jismaili@mrc.gm
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|