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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2002-1-4
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pubmed:abstractText |
IL-10 is an anti-inflammatory cytokine that has recently been shown to promote survival of neurons and glia. Here we establish that IL-10 induces phosphorylation of Stat3 on Tyr(705) and serves as a survival factor for N13 microglial cells. Recombinant IL-10 (10 ng/ml) decreases growth factor withdrawal-induced apoptosis by 50%, as assessed by TUNEL. In contrast to IL-10, IGF-I increases enzymatic activity of PI 3-kinase and causes phosphorylation on serine(473) of Akt but does not prevent microglial apoptosis. These data establish that IL-10 activates Stat3 and inhibits the mitochondrial pathway of cell death without activating the Akt cell survival pathway.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0165-5728
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-19
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11777539-Animals,
pubmed-meshheading:11777539-Apoptosis,
pubmed-meshheading:11777539-Cell Division,
pubmed-meshheading:11777539-Cell Survival,
pubmed-meshheading:11777539-Cells, Cultured,
pubmed-meshheading:11777539-Coloring Agents,
pubmed-meshheading:11777539-DNA-Binding Proteins,
pubmed-meshheading:11777539-Enzyme Activation,
pubmed-meshheading:11777539-Insulin-Like Growth Factor I,
pubmed-meshheading:11777539-Interleukin-10,
pubmed-meshheading:11777539-Mice,
pubmed-meshheading:11777539-Microglia,
pubmed-meshheading:11777539-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11777539-Phosphorylation,
pubmed-meshheading:11777539-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11777539-Proto-Oncogene Proteins,
pubmed-meshheading:11777539-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:11777539-Receptor, IGF Type 1,
pubmed-meshheading:11777539-Receptors, Interleukin,
pubmed-meshheading:11777539-Receptors, Interleukin-10,
pubmed-meshheading:11777539-STAT3 Transcription Factor,
pubmed-meshheading:11777539-Serine,
pubmed-meshheading:11777539-Tetrazolium Salts,
pubmed-meshheading:11777539-Thiazoles,
pubmed-meshheading:11777539-Trans-Activators,
pubmed-meshheading:11777539-Tyrosine
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pubmed:year |
2002
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pubmed:articleTitle |
IL-10 promotes survival of microglia without activating Akt.
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pubmed:affiliation |
Laboratory of Immunophysiology, Department of Animal Sciences, University of Illinois, 207 ERML, 1201 W. Gregory Dr., Urbana, IL 61801, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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