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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-1-4
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pubmed:abstractText |
A new fluorescence in situ hybridization (FISH) method with peptide nucleic acid (PNA) probes for identification of Staphylococcus aureus directly from positive blood culture bottles that contain gram-positive cocci in clusters (GPCC) is described. The test (the S. aureus PNA FISH assay) is based on a fluorescein-labeled PNA probe that targets a species-specific sequence of the 16S rRNA of S. aureus. Evaluations with 17 reference strains and 48 clinical isolates, including methicillin-resistant and methicillin-susceptible S. aureus species, coagulase-negative Staphylococcus species, and other clinically relevant and phylogenetically related bacteria and yeast species, showed that the assay had 100% sensitivity and 96% specificity. Clinical trials with 87 blood cultures positive for GPCC correctly identified 36 of 37 (97%) of the S. aureus-positive cultures identified by standard microbiological methods. The positive and negative predictive values were 100 and 98%, respectively. It is concluded that this rapid method (2.5 h) for identification of S. aureus directly from blood culture bottles that contain GPCC offers important information for optimal antibiotic therapy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-10449448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-10488893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-10618138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-10655393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-11069639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-11157265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-11682542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-11714518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-1939570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-2439888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-2466341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-3410948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-6771870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-7683195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-7699065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-7702812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-9542931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11773123-9650937
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11773123-Blood,
pubmed-meshheading:11773123-Culture Media,
pubmed-meshheading:11773123-Humans,
pubmed-meshheading:11773123-In Situ Hybridization, Fluorescence,
pubmed-meshheading:11773123-Nucleic Acid Probes,
pubmed-meshheading:11773123-Peptide Nucleic Acids,
pubmed-meshheading:11773123-RNA, Ribosomal, 16S,
pubmed-meshheading:11773123-Sensitivity and Specificity,
pubmed-meshheading:11773123-Staphylococcal Infections,
pubmed-meshheading:11773123-Staphylococcus aureus,
pubmed-meshheading:11773123-Time Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.
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pubmed:affiliation |
Boston Probes, Inc., Bedford, Massachusetts 01730, USA.
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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