| pubmed-article:11759685 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0030844 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0995740 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0205463 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:11759685 | lifeskim:mentions | umls-concept:C1550176 | lld:lifeskim |
| pubmed-article:11759685 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11759685 | pubmed:dateCreated | 2001-12-10 | lld:pubmed |
| pubmed-article:11759685 | pubmed:abstractText | The production of adipoyl-7-aminodeacetoxy-cephalosporanic acid (ad-7-ADCA) was studied, using two recombinant strains of Penicillium chrysogenum carrying the expandase gene from Streptomyces clavuligerus. The adipoyl-side chain of this compound may easily be removed using an amidase; and this process therefore represents a new route for the production of 7-ADCA, which serves as a precursor for the production of many semi-synthetic cephalosporins. In this study, one low- and one high-yielding strains were characterised and the specific productivities of ad-7-ADCA and byproducts of the biosynthetic pathway were compared. The fluxes through the biosynthetic pathway were quantified and it was found that there was a 30% higher flux through the expandase in the high-yielding strain. In both strains, there was a significant degradation of adipate. Furthermore, the initial adipate concentration in batch cultures was shown to have a positive effect on the formation of ad-7-ADCA. | lld:pubmed |
| pubmed-article:11759685 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11759685 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11759685 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11759685 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11759685 | pubmed:issn | 0175-7598 | lld:pubmed |
| pubmed-article:11759685 | pubmed:author | pubmed-author:NielsenJJ | lld:pubmed |
| pubmed-article:11759685 | pubmed:author | pubmed-author:BeyerMM | lld:pubmed |
| pubmed-article:11759685 | pubmed:author | pubmed-author:RobinJJ | lld:pubmed |
| pubmed-article:11759685 | pubmed:author | pubmed-author:JakobsenMM | lld:pubmed |
| pubmed-article:11759685 | pubmed:author | pubmed-author:NoormanHH | lld:pubmed |
| pubmed-article:11759685 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11759685 | pubmed:volume | 57 | lld:pubmed |
| pubmed-article:11759685 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11759685 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11759685 | pubmed:pagination | 357-62 | lld:pubmed |
| pubmed-article:11759685 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:meshHeading | pubmed-meshheading:11759685... | lld:pubmed |
| pubmed-article:11759685 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11759685 | pubmed:articleTitle | Physiological characterisation of Penicillium chrysogenum strains expressing the expandase gene from Streptomyces clavuligerus during batch cultivations. Growth and adipoyl-7-aminodeacetoxycephalosporanic acid production. | lld:pubmed |
| pubmed-article:11759685 | pubmed:affiliation | Centre for Process Biotechnology, Biocentrum-DTU, Technical University of Denmark, Lyngby. | lld:pubmed |
| pubmed-article:11759685 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11759685 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:11759685 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
