|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-12-10
|
|
pubmed:abstractText |
The production of adipoyl-7-aminodeacetoxy-cephalosporanic acid (ad-7-ADCA) was studied, using two recombinant strains of Penicillium chrysogenum carrying the expandase gene from Streptomyces clavuligerus. The adipoyl-side chain of this compound may easily be removed using an amidase; and this process therefore represents a new route for the production of 7-ADCA, which serves as a precursor for the production of many semi-synthetic cephalosporins. In this study, one low- and one high-yielding strains were characterised and the specific productivities of ad-7-ADCA and byproducts of the biosynthetic pathway were compared. The fluxes through the biosynthetic pathway were quantified and it was found that there was a 30% higher flux through the expandase in the high-yielding strain. In both strains, there was a significant degradation of adipate. Furthermore, the initial adipate concentration in batch cultures was shown to have a positive effect on the formation of ad-7-ADCA.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adipic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Cephalosporins,
http://linkedlifedata.com/resource/pubmed/chemical/Fructose,
http://linkedlifedata.com/resource/pubmed/chemical/Gluconates,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Intramolecular Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillanic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillin-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sucrose,
http://linkedlifedata.com/resource/pubmed/chemical/adipic acid,
http://linkedlifedata.com/resource/pubmed/chemical/adipoyl-7-amino-3-deacetoxycephalosp...,
http://linkedlifedata.com/resource/pubmed/chemical/aminopenicillanic acid,
http://linkedlifedata.com/resource/pubmed/chemical/deacetoxycephalosporin C synthetase,
http://linkedlifedata.com/resource/pubmed/chemical/gluconic acid
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0175-7598
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
57
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
357-62
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11759685-Adipic Acids,
pubmed-meshheading:11759685-Cephalosporins,
pubmed-meshheading:11759685-Fructose,
pubmed-meshheading:11759685-Gluconates,
pubmed-meshheading:11759685-Glucose,
pubmed-meshheading:11759685-Intramolecular Transferases,
pubmed-meshheading:11759685-Mutagenesis, Insertional,
pubmed-meshheading:11759685-Penicillanic Acid,
pubmed-meshheading:11759685-Penicillin-Binding Proteins,
pubmed-meshheading:11759685-Penicillium chrysogenum,
pubmed-meshheading:11759685-Streptomyces,
pubmed-meshheading:11759685-Sucrose
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Physiological characterisation of Penicillium chrysogenum strains expressing the expandase gene from Streptomyces clavuligerus during batch cultivations. Growth and adipoyl-7-aminodeacetoxycephalosporanic acid production.
|
|
pubmed:affiliation |
Centre for Process Biotechnology, Biocentrum-DTU, Technical University of Denmark, Lyngby.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
