Source:http://linkedlifedata.com/resource/pubmed/id/11756323
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2001-12-28
|
| pubmed:abstractText |
We investigated the capacity of human islets to produce monocyte chemoattractant protein-1 (MCP-1). Primary cultures of pancreatic islets expressed and secreted MCP-1, as determined by Northern blot, immunohistochemistry, in situ hybridization, and enzyme-linked immunosorbent assay. The produced MCP-1 was biologically active as it attracted monocytes in chemotaxis assay, and chemotactic activity was almost abrogated by a neutralizing anti-MCP-1 monoclonal antibody. Expression of MCP-1 was increased by primary inflammatory cytokines (interleukin-1 beta, tumor necrosis factor-alpha) and lipopolysaccharide at both the mRNA and protein levels but not by glucose. However, MCP-1 did not modulate insulin secretion. MCP-1 secreted by pancreatic islets plays a relevant role in the clinical outcome of islet transplant in patients with type 1 diabetes. In fact, low MCP-1 secretion resulted as the most relevant factor for long-lasting insulin independence. This finding opens new approaches in the management of human islet transplantation. Finally, the finding that MCP-1 appears constitutively present in normal human islet beta-cells (immunohistochemistry and in situ hybridization), in the absence of an inflammatory infiltrate, suggests that this chemokine could have functions other than monocyte recruitment and opens a new link between the endocrine and immune systems.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0012-1797
|
| pubmed:author |
pubmed-author:AldrighettiLucaL,
pubmed-author:AllavenaPaolaP,
pubmed-author:BertuzziFedericoF,
pubmed-author:BianchiGiancarloG,
pubmed-author:Di CarloValerioV,
pubmed-author:LeoneBiagio EugenioBE,
pubmed-author:MaffiPaolaP,
pubmed-author:MelziRaffaellaR,
pubmed-author:MontiPaoloP,
pubmed-author:NanoRitaR,
pubmed-author:PeriGiuseppeG,
pubmed-author:PiemontiLorenzoL,
pubmed-author:SaccaniAlessandraA,
pubmed-author:SecchiAntonioA,
pubmed-author:SicaAntonioA
|
| pubmed:issnType |
Print
|
| pubmed:volume |
51
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
55-65
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:11756323-Adult,
pubmed-meshheading:11756323-Blotting, Northern,
pubmed-meshheading:11756323-Cells, Cultured,
pubmed-meshheading:11756323-Chemokine CCL2,
pubmed-meshheading:11756323-Chemotaxis, Leukocyte,
pubmed-meshheading:11756323-Cytokines,
pubmed-meshheading:11756323-Diabetes Mellitus, Type 1,
pubmed-meshheading:11756323-Diabetic Nephropathies,
pubmed-meshheading:11756323-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11756323-Female,
pubmed-meshheading:11756323-Gene Expression Regulation,
pubmed-meshheading:11756323-Humans,
pubmed-meshheading:11756323-Immunosuppression,
pubmed-meshheading:11756323-Insulin,
pubmed-meshheading:11756323-Islets of Langerhans,
pubmed-meshheading:11756323-Islets of Langerhans Transplantation,
pubmed-meshheading:11756323-Kidney Transplantation,
pubmed-meshheading:11756323-Male,
pubmed-meshheading:11756323-Middle Aged,
pubmed-meshheading:11756323-Monocytes
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation.
|
| pubmed:affiliation |
Laboratory of Experimental Surgery, Surgical Department, S. Raffaele Scientific Institute, Via Olgettina, Milan, Italy. University of Milano Bicocca, Milan, Italy. piemonti.lorenzo@hsr.it
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|