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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1975-12-30
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pubmed:abstractText |
HeLa cells were synchronized with a double thymidine block. Ribosomal subunits, monomers and polyribosomes have been quantitatively analysed at hourly intervals, during interphase, and every 15 min, during mitosis. This analysis was performed on linear 7-47% sucrose gradients. From the beginning of G1 up to the end of S phase, a certain equilibrium among ribosomal subunits, monomers and polyribosomes is maintained, while from the time of entering G2 to M the translation machinery appears to be mobilized in the sense of polysome formation. Under these conditions, the amount of polysomes per cell during the mitotic cycle is expressed by a bi-phasic pattern showing pre- and post-mitotic peaks with a falling-off during S. The G1 peak, meanwhile, is much lower than the G2 peak. The incorporation of [3H]leucine into nascent polypeptide chains on polysomes, as well as into bulk cell proteins and into nuclear and cytoplasmic proteins considered separately, is also represented by a bi-phasic curve which shows, however, a higher peak in G1 and a lower peak in G2, with two fallings-off during S and M, respectively. Since between the G1 and the G2 amino acid pools there are not strong differences of leucine concentration, the discrepancy between the amount of polysomes and the rate of labelling is discussed on the basis of the differences of polysome shape found at the different stages of the cycle. In young cells, in fact, there is an abundance of small polysomes, while in the old cell large polysomes predominate. It is suggested that, in the old cell, the rate of translation on large polysomes could be relatively lower or that among these heavy aggregates a given number of "frozen" polysomes could be present. The ribosome state is considered as a probable limiting-factor of translation, particularly in mitosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
203-10
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:1175585-Cell Division,
pubmed-meshheading:1175585-Cell Nucleus,
pubmed-meshheading:1175585-Cytoplasm,
pubmed-meshheading:1175585-HeLa Cells,
pubmed-meshheading:1175585-Leucine,
pubmed-meshheading:1175585-Neoplasm Proteins,
pubmed-meshheading:1175585-Nucleoproteins,
pubmed-meshheading:1175585-Polyribosomes,
pubmed-meshheading:1175585-Protein Biosynthesis,
pubmed-meshheading:1175585-Ribosomes,
pubmed-meshheading:1175585-Time Factors
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pubmed:year |
1975
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pubmed:articleTitle |
Polysome translational state during the cell cycle.
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pubmed:publicationType |
Journal Article
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