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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-12-25
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pubmed:abstractText |
The effect of quercetin on lipopolysaccharide (LPS)-induced nitric oxide (NO) production was studied. Quercetin pretreatment significantly inhibited NO production in an LPS-stimulated RAW 264.7 murine macrophage cell line. Post-treatment with quercetin partially inhibited NO production. The inhibitory action of quercetin was due to neither the cytotoxic action nor altered LPS binding. The expression of inducible-type NO synthase (iNOS) was markedly down-regulated by quercetin. Quercetin suppressed the release of free nuclear factor (NF)-kappaB by preventing degradation of IkappaB-alpha and IkappaB-beta. Moreover, quercetin blocked the phosphorylation of extracellular signal regulated kinase 1/2 (Erk 1/2), p38, and c-Jun NH2-terminal kinase/stress-activated protein kinase (JNK/SAPK) and, further, the activity of tyrosine kinases in LPS-stimulated RAW cells. Quercetin also inhibited interferon (IFN)-gamma-induced NO production. Taken together, these results indicate that the inhibitory action of quercetin on NO production in LPS- and/or IFN-gamma-stimulated macrophages might be due to abrogation of iNOS protein induction by impairment of a series of intracellular signal pathways.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 8,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Quercetin,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:issn |
0968-0519
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
431-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11753212-Animals,
pubmed-meshheading:11753212-Antioxidants,
pubmed-meshheading:11753212-Cell Line,
pubmed-meshheading:11753212-Down-Regulation,
pubmed-meshheading:11753212-Enzyme Induction,
pubmed-meshheading:11753212-Enzyme Inhibitors,
pubmed-meshheading:11753212-I-kappa B Proteins,
pubmed-meshheading:11753212-Immunoblotting,
pubmed-meshheading:11753212-Interferon-gamma,
pubmed-meshheading:11753212-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:11753212-Lipopolysaccharides,
pubmed-meshheading:11753212-Macrophages,
pubmed-meshheading:11753212-Mice,
pubmed-meshheading:11753212-Mitogen-Activated Protein Kinase 8,
pubmed-meshheading:11753212-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:11753212-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11753212-NF-kappa B,
pubmed-meshheading:11753212-Nitric Oxide,
pubmed-meshheading:11753212-Nitric Oxide Synthase,
pubmed-meshheading:11753212-Nitric Oxide Synthase Type II,
pubmed-meshheading:11753212-Phosphorylation,
pubmed-meshheading:11753212-Quercetin,
pubmed-meshheading:11753212-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2001
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pubmed:articleTitle |
The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells.
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pubmed:affiliation |
Department of Microbiology and Immunology and Division of Bacterial Toxin, Research Center for Infectious Disease, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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