Linked Life Data

11752788

Source:http://linkedlifedata.com/resource/pubmed/id/11752788

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2001-12-25
pubmed:abstractText
A metastasis-inducing protein, human S100A4, cDNA was overexpressed in Escherichia coli. The recombinant protein was purified by three chromatographic procedures. Crystals were obtained using polyethylene glycol as a precipitant. The native S100A4 crystals did not diffract sufficiently well for data collection, but the SeMet S100A4 crystal, grown using similar conditions as for the native, was found to be stable during exposure to X-rays and diffracted to 4 A resolution in-house. The crystal belongs to space group P6 or P3. The unit-cell parameters are a = b = 47.1, c = 175.6 A, alpha = beta = 90, gamma = 120 degrees. Complete data will be collected using synchrotron radiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-9
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Crystallization and preliminary crystallographic analysis of a metastasis-inducing protein, human S100A4.
pubmed:affiliation
Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Life Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't