Source:http://linkedlifedata.com/resource/pubmed/id/11748919
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0007586,
umls-concept:C0013935,
umls-concept:C0016030,
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0025548,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0127400,
umls-concept:C0178638,
umls-concept:C0185117,
umls-concept:C0205112,
umls-concept:C0205251,
umls-concept:C0441889,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C2349975,
umls-concept:C2911684
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| pubmed:issue |
12
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| pubmed:dateCreated |
2001-12-25
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| pubmed:abstractText |
The impact of basal (non-induced) expression levels of metallothionein I and II on the growth of mouse embryo fibroblasts in standard DMEM/F-12 containing 8.8 microm folic acid, and in DMEM/F12 without hypoxanthine, thymidine or folic acid, containing 15 nm or 15 pm[6S]-folinic acid, was assessed by comparing wild-type MT (+/+) and homozygous null MT (-/-) cell lines. No difference in growth rate was observed between the two in DMEM/F12, although MT (-/-) cells displayed a 6-fold decrease in p27(Kip1), a two fold increase in p53 and a slight increase in p21(Waf1). After 6 days in culture, the growth rate for MT (-/-) cells in 15 nm or 15 pm[6S]-folinic acid was half that of MT (+/+). After an additional 6 days in 15 n m folate, both MT (+/+) and (-/-) cells maintained their respective growth rates, while those in 15 pm had ceased to grow. During the initial 6 days in 15 nm folate, neither cell population displayed an increase in apoptosis or a change in cell cycle distribution, even though MT (-/-) cells sustained an additional 4-fold increase in p21(Waf1)and a 6-fold decrease in cyclin E expression. At day 12, however, the MT (-/-) population, but not MT (+/+), underwent a 7-fold increase in apoptosis coupled with a 3 fold increase in S phase cells. Hence, the basal level of MT I and II constitutively expressed in MT (+/+) cells enhances growth in 15nM [6S]-folinic acid by preventing S phase arrest and apoptosis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1065-6995
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:volume |
25
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1261-9
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11748919-Animals,
pubmed-meshheading:11748919-Blotting, Northern,
pubmed-meshheading:11748919-Blotting, Western,
pubmed-meshheading:11748919-Cell Cycle,
pubmed-meshheading:11748919-Cells, Cultured,
pubmed-meshheading:11748919-Cyclins,
pubmed-meshheading:11748919-Dose-Response Relationship, Drug,
pubmed-meshheading:11748919-Embryo, Mammalian,
pubmed-meshheading:11748919-Fibroblasts,
pubmed-meshheading:11748919-Flow Cytometry,
pubmed-meshheading:11748919-Folic Acid,
pubmed-meshheading:11748919-In Situ Nick-End Labeling,
pubmed-meshheading:11748919-Metallothionein,
pubmed-meshheading:11748919-Mice,
pubmed-meshheading:11748919-Mice, Knockout,
pubmed-meshheading:11748919-RNA, Messenger
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| pubmed:year |
2001
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| pubmed:articleTitle |
Basal levels of metallothionein I and II expression in mouse embryo fibroblasts enhance growth in low folate through a cell cycle mediated pathway.
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| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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