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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-12-18
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pubmed:abstractText |
Bone morphogenetic proteins play important roles in connective tissue morphogenesis. In this study, we used human multipotential mesenchymal cells as a target to analyze the effect of bone morphogenetic proteins on chondrogenesis. We also analyzed the effect of proinflammatory cytokine interleukin-1 on chondrogenic-differentiated cells and the interaction of IL-1beta with bone morphogenetic proteins. Cells placed in a 3-dimensional matrix of alginate beads and cultured in a serum-free media with bone morphogenetic protein-2 and -9 induced expression of type II collagen (Col2A1) mRNA and increased expression of aggrecan and cartilage oligomeric matrix protein suggesting chondrogenic differentiation of the cells. The transcription factor Sox-9 that regulates both Col2A1 and aggrecan gene expression showed increased expression with BMP treatment. Chondrogenic differentiated cells treated with interleukin-1 decreased Sox-9, Col2A1 and aggrecan gene expression. Removal of interleukin-1 and further addition of bone morphogenetic proteins resulted in returned expression of chondrogenic markers. Chondrogenic differentiated cells cultured in the presence of different concentrations of bone morphogenetic proteins and interleukin-1 showed that bone morphogenetic proteins were able to partially block the suppressive effect of interleukin-1. This study shows that bone morphogenetic proteins play an important role in chondrogenesis and may prove to be potential therapeutics in cartilage repair.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aggrecans,
http://linkedlifedata.com/resource/pubmed/chemical/BMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Differentiation Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Differentiation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SOX9 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/SOX9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9541
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
189
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-84
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11748585-Aggrecans,
pubmed-meshheading:11748585-Biological Markers,
pubmed-meshheading:11748585-Bone Morphogenetic Protein 2,
pubmed-meshheading:11748585-Bone Morphogenetic Proteins,
pubmed-meshheading:11748585-Cell Differentiation,
pubmed-meshheading:11748585-Cells, Cultured,
pubmed-meshheading:11748585-Chondrocytes,
pubmed-meshheading:11748585-Chondrogenesis,
pubmed-meshheading:11748585-Collagen Type II,
pubmed-meshheading:11748585-Drug Antagonism,
pubmed-meshheading:11748585-Extracellular Matrix Proteins,
pubmed-meshheading:11748585-Growth Differentiation Factor 2,
pubmed-meshheading:11748585-Growth Differentiation Factors,
pubmed-meshheading:11748585-High Mobility Group Proteins,
pubmed-meshheading:11748585-Humans,
pubmed-meshheading:11748585-Interleukin-1,
pubmed-meshheading:11748585-Kinetics,
pubmed-meshheading:11748585-Lectins, C-Type,
pubmed-meshheading:11748585-Mesoderm,
pubmed-meshheading:11748585-Proteoglycans,
pubmed-meshheading:11748585-RNA, Messenger,
pubmed-meshheading:11748585-SOX9 Transcription Factor,
pubmed-meshheading:11748585-Stem Cells,
pubmed-meshheading:11748585-Transcription Factors,
pubmed-meshheading:11748585-Transforming Growth Factor beta
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pubmed:year |
2001
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pubmed:articleTitle |
BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1.
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pubmed:affiliation |
Genetics Institute, Inc., Cambridge, Massachusetts 02140, USA. mmajumdar@genetics.com
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pubmed:publicationType |
Journal Article
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