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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2001-12-18
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pubmed:abstractText |
To elucidate the host cell defense mechanisms in response to Sindbis viral infection, we have started to characterize interferon (IFN)-stimulated response element (ISRE)-binding proteins activated in infected cells that are involved in the transcriptional induction of IFN type I-inducible genes. Using electromobility shift assays (EMSA), we detected several protein complexes with a human IFN-stimulated gene 15 (ISG15) ISRE in extracts from virus-infected L929 cells that were absent in extracts from uninfected cells. Comigration with Newcastle disease virus-activated ISRE-binding complexes, ISRE-binding specificity, supershift experiments, and conditions of formation indicate that the complexes activated by Sindbis viral infection in L929 cells correspond to DRAF1 and ISG factor 3 (ISGF3). Transfection of L929 cells with poly rI:rC induced only ISGF3. DRAF1 could be detected in Sindbis virus-infected mouse embryo fibroblasts derived from IFNR type I and type II KO mice. Viral RNA synthesis is required for activation of DRAF1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DRAF1 transcription factor,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/G1p2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1079-9907
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
981-90
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11747630-Animals,
pubmed-meshheading:11747630-Cell Line,
pubmed-meshheading:11747630-Cercopithecus aethiops,
pubmed-meshheading:11747630-Cytokines,
pubmed-meshheading:11747630-DNA-Binding Proteins,
pubmed-meshheading:11747630-Dactinomycin,
pubmed-meshheading:11747630-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:11747630-Interferon Type I,
pubmed-meshheading:11747630-Macromolecular Substances,
pubmed-meshheading:11747630-Mice,
pubmed-meshheading:11747630-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:11747630-RNA, Double-Stranded,
pubmed-meshheading:11747630-RNA, Messenger,
pubmed-meshheading:11747630-RNA, Viral,
pubmed-meshheading:11747630-Response Elements,
pubmed-meshheading:11747630-Sindbis Virus,
pubmed-meshheading:11747630-Transcription Factors,
pubmed-meshheading:11747630-Transcriptional Activation,
pubmed-meshheading:11747630-Ubiquitins,
pubmed-meshheading:11747630-Vero Cells
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pubmed:year |
2001
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pubmed:articleTitle |
Interferon-stimulated response element (ISRE)-binding protein complex DRAF1 is activated in Sindbis virus (HR)-infected cells.
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pubmed:affiliation |
Institute for Virology and Immunology, University of Würzburg, 97078 Würzburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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