Linked Life Data

11746778

Source:http://linkedlifedata.com/resource/pubmed/id/11746778

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-12-17
pubmed:abstractText
Glial cell line-derived neurotrophic factor (GDNF) plays several important roles in the survival and recovery of mature neurons during ischemia. We examined the possibility that the expression of GDNF mRNA and the release of GDNF protein are regulated differentially in cultured astrocytes from the stroke-prone spontaneously hypertensive rat (SHRSP) compared with those from Wistar Kyoto rats (WKY) during hypoxia and reoxygenation (H/R) and after exposure to glutamate and hydrogen peroxide (H(2)O(2)). The mRNA expression was quantitated by reverse transcription-polymerase chain reaction (RT-PCR) based on the fluorescent TaqMan methodology. A new instrument capable of measuring fluorescence in real-time was used to quantify gene amplification in astrocytes. GDNF protein was investigated by enzyme-linked immunosorbent assay (ELISA). GDNF mRNA expression and GDNF protein release at normoxia were greater in SHRSP than in WKY astrocytes. During H/R, however, the mRNA expression and protein release tended to be reduced in SHRSP compared with WKY. Glutamate and H(2)O(2) induced the expression of GDNF mRNA and the release of GDNF protein in both WKY and SHRSP in a dose-dependent manner. Levels of GDNF mRNA and protein in SHRSP were significantly lower than in WKY. These findings indicate that GDNF production in SHRSP astrocytes was low in response to H/R, glutamate, and H(2)O(2), compared with that observed in WKY. We conclude that the attenuated production of GDNF in astrocytes is involved in neuronal vulnerability in SHRSP during H/R, as GDNF production, which is stimulated by glutamate and H(2)O(2), is closely related to the protective effect against H/R-mediated neurotoxicity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0894-1491
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11746778-Animals, pubmed-meshheading:11746778-Astrocytes, pubmed-meshheading:11746778-Brain, pubmed-meshheading:11746778-Cells, Cultured, pubmed-meshheading:11746778-Dose-Response Relationship, Drug, pubmed-meshheading:11746778-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11746778-Fetus, pubmed-meshheading:11746778-Gene Expression Regulation, pubmed-meshheading:11746778-Glial Cell Line-Derived Neurotrophic Factor, pubmed-meshheading:11746778-Glutamic Acid, pubmed-meshheading:11746778-Hydrogen Peroxide, pubmed-meshheading:11746778-Hypoxia-Ischemia, Brain, pubmed-meshheading:11746778-Nerve Growth Factors, pubmed-meshheading:11746778-Nerve Tissue Proteins, pubmed-meshheading:11746778-RNA, Messenger, pubmed-meshheading:11746778-Rats, pubmed-meshheading:11746778-Rats, Inbred SHR, pubmed-meshheading:11746778-Rats, Inbred WKY, pubmed-meshheading:11746778-Reperfusion Injury, pubmed-meshheading:11746778-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2002
pubmed:articleTitle
Differential regulation of glial cell line-derived neurotrophic factor (GDNF) mRNA expression during hypoxia and reoxygenation in astrocytes isolated from stroke-prone spontaneously hypertensive rats.
pubmed:affiliation
Division of Life Science, Graduate School of Integrated Science and Art, University of East Asia, Yamaguchi, Japan. yamagata@po.cc.toua-u.ac.jp
pubmed:publicationType
Journal Article, Comparative Study