Source:http://linkedlifedata.com/resource/pubmed/id/11745349
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2001-12-17
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pubmed:abstractText |
Keratocytes express MHC class I molecules constitutively, and keratocytes stimulated with IFN-gamma express MHC class II molecules. Unstimulated keratocytes constitutively express B7-1 and ICAM-1, as well as low levels of CD40 and 4-1BBL. These findings indicate that keratocytes may deliver both antigen-specific and costimulatory signals to CD4(+) and CD8(+) T cells. To demonstrate that keratocytes expressing B7-1 provide a costimulatory signal to T cells, CD4(+) or CD8(+) mouse T cells were incubated with anti-CD3 mAb and irradiated keratocytes. Enhanced proliferation of both CD4(+) and CD8(+) T cells occurred, and could be inhibited by anti-B7-1 mAb, indicating T cell costimulatory activity by B7-1 on the keratocytes. To demonstrate that keratocytes can deliver an antigen-specific signal, CD4(+) and CD8(+) T cells from herpes-infected mice were incubated with HSV-1-infected, irradiated keratocytes. The resulting T cell proliferation and production of Th1 cytokines (IL-2, IFN-gamma) indicated T cell activation by antigens presented by the infected keratocytes. These results show that keratocytes in the corneal stroma of the mouse can function as antigen-presenting cells and, thus, may play a role in immune-mediated stromal inflammation such as herpetic stromal keratitis.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3318-28
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11745349-Animals,
pubmed-meshheading:11745349-Antigen-Presenting Cells,
pubmed-meshheading:11745349-Antigens, CD,
pubmed-meshheading:11745349-Antigens, CD40,
pubmed-meshheading:11745349-Antigens, CD80,
pubmed-meshheading:11745349-Antigens, CD86,
pubmed-meshheading:11745349-Cells, Cultured,
pubmed-meshheading:11745349-Cornea,
pubmed-meshheading:11745349-Cytokines,
pubmed-meshheading:11745349-Female,
pubmed-meshheading:11745349-Histocompatibility Antigens Class II,
pubmed-meshheading:11745349-Keratitis,
pubmed-meshheading:11745349-Lymphocyte Activation,
pubmed-meshheading:11745349-Membrane Glycoproteins,
pubmed-meshheading:11745349-Mice,
pubmed-meshheading:11745349-Mice, Inbred BALB C,
pubmed-meshheading:11745349-Stromal Cells
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pubmed:year |
2001
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pubmed:articleTitle |
Murine keratocytes function as antigen-presenting cells.
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pubmed:affiliation |
The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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