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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-12-17
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pubmed:abstractText |
The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na(+)-HCO(3)(-) cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO(3)(-) uptake via a Na(+)-dependent, DIDS-sensitive mechanism. By reverse transcriptase-polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na(+)- and HCO(3)(-)-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na(+)-HCO(3)(-) cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Ammonium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Diuretics,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/SLC4A4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Bicarbonate Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0003-9969
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11743927-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:11743927-Adult,
pubmed-meshheading:11743927-Aged,
pubmed-meshheading:11743927-Amiloride,
pubmed-meshheading:11743927-Ammonium Chloride,
pubmed-meshheading:11743927-Animals,
pubmed-meshheading:11743927-Cell Membrane,
pubmed-meshheading:11743927-Cloning, Molecular,
pubmed-meshheading:11743927-Cytophotometry,
pubmed-meshheading:11743927-Diuretics,
pubmed-meshheading:11743927-Female,
pubmed-meshheading:11743927-Gene Expression,
pubmed-meshheading:11743927-Humans,
pubmed-meshheading:11743927-Hydrogen-Ion Concentration,
pubmed-meshheading:11743927-Immunohistochemistry,
pubmed-meshheading:11743927-Male,
pubmed-meshheading:11743927-Membrane Potentials,
pubmed-meshheading:11743927-Microelectrodes,
pubmed-meshheading:11743927-Middle Aged,
pubmed-meshheading:11743927-Oocytes,
pubmed-meshheading:11743927-Parotid Gland,
pubmed-meshheading:11743927-Protein Isoforms,
pubmed-meshheading:11743927-Sodium,
pubmed-meshheading:11743927-Sodium-Bicarbonate Symporters,
pubmed-meshheading:11743927-Sodium-Hydrogen Antiporter,
pubmed-meshheading:11743927-Statistics as Topic,
pubmed-meshheading:11743927-Xenopus laevis
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pubmed:year |
2002
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pubmed:articleTitle |
Expression of a sodium bicarbonate cotransporter in human parotid salivary glands.
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pubmed:affiliation |
School of Biological Sciences, University of Manchester, G.38 Stopford Building, M13 9PT, Manchester, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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